In: Biology
Given tetrapeptide is Val- Ala-Gly-Leu, Use the strand genetic code abbreviations supplemented with the ambiguous code: N=any nucleotide; Pu= a [urine; Py= a pyrimidine; if one of two nucleotides that are not both Py or Pu, use e.g C/G. Separate each codon with a vertical line e.g 5’-NCG|PyPuG|.
tetrapeptide is Val- Ala-Gly-Leu
Answer is----->codon are- 5' PuPyN / PuPyN / PuPuN / PyPyN 3'.
Need an explanation with clear process please.!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
The tetrapeptide whose genetic code needs to be written is: Val-Ala-Gly-Leu
Let us see the table of three-letter combinations of nucleotides/codon which code for each of these amino acids:
1. Valine: GUC, GUA, GUG, GUU
Therefore, with the ambiguous code, the codons can be represented as: PuPYN
Here, Pu= Guanine, Py = Uracil and N= Any nucleotide (except thymidine)
2. Alanine: GCU, GCC, GCA, GCG
Therefore, with the ambiguous code, the codons can be represented as: PuPYN
Here, Pu= Guanine, Py = cytosine and N= Any nucleotide (except thymidine)
3. Glycine: GGU, GGG, GGA, GGC
Therefore, with the ambiguous code, the codons can be represented as: PuPuN
Here, Pu= Guanine, Pu = Guanine and N= Any nucleotide (except thymidine)
4. Leucine: CUU, CUA, CUG, CUC
Therefore, with the ambiguous code, the codons can be represented as: PyPyN
Here, Py= Cytosine, Py = Uracil and N= Any nucleotide (except thymidine)
Now, the answer will be to join these four ambiguous codes and add a 5' at the beginning and 3' at the end, with each codon separated by a vertical line.
Thus the answer is: 5' PuPyN / PuPyN / PuPuN / PyPyN 3'