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In: Advanced Math

You are developing primers for a wildlife forensic case and want to identify both species and...

You are developing primers for a wildlife forensic case and want to identify both species and distinguish between individuals. a) For which type of analysis would you want to develop/use degenerate primers? Why? b) If the primer you developed for species ID is 14bp, the mitochondrial genome is 18500 bp, and the nuclear genome is 3.2x109 bp, how many times would you expect it to bind to each of the respective genomes assuming the same primer could bind to both the mtDNA and nDNA genomes? (2marks) c) Comment on the specificity of the primer if this primer was intended to amplify only a mtDNA region and not a nDNA region.

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Colby Messinger answered 2 years ago
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