why do we need to calculate the titer of bacteriophage? what does it tell you? what...

why do we need to calculate the titer of bacteriophage? what does it tell you? what titer is considered high/low?

Expert Solution

One of the most important procedures in virology is measuring the virus titer- the concentration of viruses in a sample. It is used for determining the quantity of infectious virus and the widely used method to calculate the titers of bacteriophage stocks is plaque assay. This method helps in pure isolation of bacteriophage populations and its purification,and to optimise the viral titers. The basis of plaque assay is to measure the ability of a single infectious virus to form a plaque on a concurrent monolayer culture cells.

The titer of a virus stock can be calculated in plaque-forming units (PFU) per millimeter. This calculation describes the number of virus particles capable of forming plaques per unit volume. It is a proxy measurement of quantity of viral particles that are defective or which fail to infect their target cell to produce plaque and thus will not be counted. It is based on the assumption that each plaque formed represents one infective virus particles.

Lysates with a final concentration greater than 109PFU/ml are "High Titer" lysates.

gladiator answered 2 years ago

1. Why do we need a statement of retained earnings?What does it tell stakeholders?2....

1. Why do we need a statement of retained earnings? What does it tell stakeholders?2. Temporary accounts, permanent accounts, closing them, not closing them, I'm confused. Explain this to me.3. Why would a company WANT to have a high current ratio? What does it signify

What does a confidence interval tell us and how do we calculate one? As part of...

What does a confidence interval tell us and how do we calculate one? As part of your answer, please come up with an example.

Can you tell me why in some experiments (such as biodiesel production) do we need to...

Can you tell me why in some experiments (such as biodiesel production) do we need to test the cloud point and pour point? What is the purpose?

why do we need to calculate the cpi and what is the basket of consumers good...

why do we need to calculate the cpi and what is the basket of consumers good and services?

3. What does the test statistic tell us? 4. Why do we divide by the standard...

3. What does the test statistic tell us? 4. Why do we divide by the standard error when computing a test statistic? 5. Why do we reject the null hypothesis when the p-value is small? Explain as if to someone unfamiliar with statistics.

Do we need Tort Reform? If not, why not? If so, what reform do you like...

Do we need Tort Reform? If not, why not? If so, what reform do you like the best?

In regression results. what does the R-squared value tell you? What do the coefficients tell you?...

In regression results. what does the R-squared value tell you? What do the coefficients tell you? What do the P-values tell you? What does a Q-Q plot tell you? What do residuals tell you?

Intuitively, what is the discount rate, why do we need it, and how do we choose...

Intuitively, what is the discount rate, why do we need it, and how do we choose the appropriate number?

Why is it important to plan communications? Why do we need a strategy? How does new...

Why is it important to plan communications? Why do we need a strategy? How does new media factor in our communication strategy? What is the risk to not having an integrated strategy? Remember you must support your response with ate least two scholarly sources. Don’t forget to use APA style in-text citations and references.

What does Operating and Cash Cycles tell you? Why are they important?

Question