Question

In: Biology

If the rate of product formation is 33μM/min at a substrate concentration of 10 mM, and...

If the rate of product formation is 33μM/min at a substrate concentration of 10 mM, and the enzyme is 50% saturated at a substrate concentration of 20mM, the Vmax of the enzyme is: :

A. 10μM/min B. 33μM/min C. 67μM/min D. 99μM/min E. 30mM/min

Solutions

Expert Solution

The correct option is D (99 µM/min)

As per Michaelis menten equation , The rate of a reaction can be calculated by the following formula

V= Vmax[s]/Km+S

Where Vmax is maximum velocity of reaction- Which has to be calculated

[S]- substrate concentration= 10 mM (Given)

Km- The substrate concentration when reaction attains half of it’s maximum velocity. In other words the concentration when 50% of enzyme is saturated with substrate= 20mM (Given).

V= initial velocity of reaction = 33µM/min

Now, let us put these values in the equation

We should change the mM units into µM by multiplying with 103

V= Vmax[s]/Km+S

33µM/min= Vmax X [10x103µM] / 20x 103 µM+10x103µM

Now we will do the cross multiplication

Vmax X [10x103µM]= 33µM/min X 20x 103 µM + 33µM/min X 10x103µM

Vmax X [10x103µM]= 660 x 103 µM /min+ 330 x 103 µM/min

Vmax X [10x103µM]=990 x103 µM/min

Vmax= 990 x103 µM / 10x103µM] (Division)

Vmax= 99 µM/min

Hence the correct option is D.

other options like A,B,C and E are incorrect.

gladiator answered 2 years ago
Next > < Previous

Related Solutions

If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate? A.Increasing enzyme...
If the substrate concentration is limiting, how does increasing enzyme concentration affect reaction rate? A.Increasing enzyme concentration has no effect on reaction rate. B.Increasing enzyme concentration results in an increase in reaction rate. C.Increasing enzyme concentration decreases reaction rate.
Sample Rate of CO2 production (mm/min) Fructose 2.65mm/min Glucose 3.3mm/min Sucrose 2.05mm/min Starch .85mm/min Water 0...
Sample Rate of CO2 production (mm/min) Fructose 2.65mm/min Glucose 3.3mm/min Sucrose 2.05mm/min Starch .85mm/min Water 0 mm/min Explain these results. Why did glucose result in the highest rate? Why did sucrose result in a lower rate than fructose, and starch even lower? Recall that sucrose is a disaccharide composed of glucose and fructose, and that starch is a polysaccharide composed of many glucose molecules.
An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km...
An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km from these measurements. (Hint: Slope = rise/run and remember what Km represents!) vo(nM/s)           [S] (nM) 30                           100,000 29.5                        10,000 29                           1,000 28                           200                         25                           150 20                           100 10                           50 5                              20 a. Vmax = 10 nM/s, Km = 20 nM b. Vmax = 20 nM/s , Km = 50 nM c. Vmax = 40 nM/s , Km = 150 nM d. Vmax =...
1a) what is the relationship between substrate concentration and reaction rate? b) Describe how the enzyme...
1a) what is the relationship between substrate concentration and reaction rate? b) Describe how the enzyme structure is effected: i) below the optimal temperature ii)above the optimal temperature?   c)Why is enzyme activity reduced at pH values above or below the optimum? How does the enzyme structure change at non-optimum pH?
Which of the following statements about enzyme substrate complexes is TRUE? A. At high substrate concentration...
Which of the following statements about enzyme substrate complexes is TRUE? A. At high substrate concentration the rate of the reaction is first order. B. The enzyme concentration is higher than the substrate concentration under physiological conditions. C. Alteration in the conformation of the enzyme may occur when substrate binds. D. Increase in enzyme concentration does not affect the rate of the reaction.
Describe the process of the formation and concentration of urine.
Describe the process of the formation and concentration of urine.
Use the following table to answer the questions Ion Extracellular Concentration (mM) Intracellular Concentration (mM) Na+...
Use the following table to answer the questions Ion Extracellular Concentration (mM) Intracellular Concentration (mM) Na+ 440 50 K+ 20 400 Cl- 560 52 Ca++ 10 1 1. What is the effect of addition of extracellular TTX and TEA on equilibrium potential? (1 points) 2. If one reduced the extracellular concentration of Na+ around a neuron, what would happen to the membrane potential? (1 point) 3. If one increased the extracellular concentration of K+ around a neuron, what would happen...
Use the following table to answer the questions Ion Extracellular Concentration (mM) Intracellular Concentration (mM) Na+...
Use the following table to answer the questions Ion Extracellular Concentration (mM) Intracellular Concentration (mM) Na+ 440 50 K+ 20 400 Cl- 560 52 Ca++ 10 1 Calculate the equilibrium potential for Na+, K+, Ca++, and Cl- ions? (4 points) If the resting membrane potential is -65, give the direction of the flow of Na+, Ca++, K+, and Cl- ion. (4 points) What will happen to the Cl- equilibrium potential, if one changes the extracellular chloride concentration from 560 mM...
Why are Km values of enzymes typically at or near the cellular substrate concentration?
Why are Km values of enzymes typically at or near the cellular substrate concentration?
BUFFERS: (Final concentration for 1x is 20 mM Tris; 500 mM NaCl; 1 mM CaCl2; 1...
BUFFERS: (Final concentration for 1x is 20 mM Tris; 500 mM NaCl; 1 mM CaCl2; 1 mM MgCl2; pH 7.4) 5x ConA Buffer, (1L)     •       700 ml dH2O, set stirring and add:     •       100 ml 1M Tris, pH 7.2-7.4     •       5 ml 1M MgCl2     •       5 ml 1M CaCl2     •       146 g NaCl     •       Check pH is between 7.2 and 7.4     •       To 1000 ml with dH2O Elution Buffer A: ~0.2M AMM: 0.1...
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT