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In: Biology

what is the diffrecence between choice test and non-choice test in entomology? explain where it happen?

what is the diffrecence between choice test and non-choice test in entomology? explain

where it happen?

Solutions

Expert Solution

No-choice tests are generally thought to maximize the expression of host range, no-choice tests should only be used with caution as they have the potential to produce false negative results. No-choice tests present the potential biological control agent with one non-target test species at a time. Thus if 10 non-target species are to be tested, there will be a series of ten cages (with replicates for each), plus appropriate controls

Choice tests including the target host have the potential to mask the acceptability of lower ranked hosts, thereby producing false negative results.In choice tests, two or more host species are presented to the test insect simultaneously and thus the response is a measure of preference for one species in the presence of another species. In the context of non-target risk assessment for biological control, the comparison between the target host and a single non-target host is usually more straight forward than a multiple choice situation. It is generally expected that host preferences will be more clearly expressed by parasitoids in choice tests compared to in no-choice tests.

No-choice tests remain the most useful method for assessing host acceptance behaviour of parasitoids. As the duration of no-choice tests increases, the potential for time-dependent effects to act upon the parasitoid will increase. Similarly because of time-dependent effects, sequential no-choice tests should be attempted with caution as false negative results can occur when the period of exposure to non-targets is too short. In choice tests, host experience may have a significant influence on the expression of host preference. Exposure to the host or plant-host complex at eclosion, even without actual oviposition experience, can bias host preference towards the natal host, obscuring acceptance of lower ranked hosts. This should be minimized by collecting the parasitoids immediately or soon after eclosion. The presentation of the hosts during the test itself (on an inert substrate, on the same host plant, or on different host plants) may overcome the potential effects of experience.

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