Question

draw a picture of what you think recombinant plasmid looks like. in your drawing, show the approximate location of any antibiotic resistant gene, the gene of interest, the restriction of endonuclease cutting sites

Answer 1

Please refer to the diagram below gor the recombinant plasmid

The above diagram shows the formation of a recombinant plasmid taking pBR322 as a vector.

The important features of the vector are:

i. ori: origin of replication

This gene is required for the replication of the plasmid

ii. rop: replication of plasmid

This gene encodes protein which facilitates the replication of the plasmid

iii. Antibiotic resistance genes: ampicillin resistance (ampr) and tetracyclin resistance (tetr) for selection of bacteria containing the recombinant plasmid on the appropriate antibiotic plate

iv. Restriction enzyme sites: PvuI, PstI, EcoRI, BamHI, SalI, HindIII, ClaI, PvuII

These restriction enzyme sites are used for insertion of the foreign DNA into the plasmid.

In the above example, BamHI and SalI sites within the tetr gene have been used to insert the foreign DNA and form the recombinant plasmid.