You have evaluated the theoretical pI of Our Protein Of Interest (OPOI), based on its amino...

You have evaluated the theoretical pI of Our Protein Of Interest (OPOI), based on its amino acid sequence, to be 6.4. You select a working pH of 8.0 and will use what type of ion exchange?______________________________

Expert Solution

queen_honey_blossom answered 1 year ago

Molecular weight: 93910.16 Theoretical pI: 6.03 1. 1. Based on the pI of Taq, what pH...

Molecular weight: 93910.16 Theoretical pI: 6.03 1. 1. Based on the pI of Taq, what pH buffer would you have to use to get Taq polymerase to stick to an anion exchanger? Briefly discuss the advantages and disadvantages of using a salt or pH in eluting proteins from an ion-exchange column. Please place details explaining how you arrived to ech answer for question 1

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Even if you see ALL OF THE SUBJECTS in our theoretical blood glucose study have LOWER blood glucose after they take the medication, does that mean that the blood glucose worked? What other factors could be influencing a lower blood glucose among all the participants? Would those factors be confounders? Effect mediators? If you had to take into consideration all of those other factors, would you still do a t test?

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Why is a protein more soluble when the pH is greater than or less than its pI?

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