You have evaluated the theoretical pI of Our Protein Of Interest (OPOI), based on its amino...

You have evaluated the theoretical pI of Our Protein Of Interest (OPOI), based on its amino acid sequence, to be 6.4. You select a working pH of 8.0 and will use what type of ion exchange?______________________________

Solutions

Expert Solution

queen_honey_blossom answered 6 months ago

Next > < Previous

Related Solutions

Molecular weight: 93910.16 Theoretical pI: 6.03 1. 1. Based on the pI of Taq, what pH...

Molecular weight: 93910.16 Theoretical pI: 6.03 1. 1. Based on the pI of Taq, what pH buffer would you have to use to get Taq polymerase to stick to an anion exchanger? Briefly discuss the advantages and disadvantages of using a salt or pH in eluting proteins from an ion-exchange column. Please place details explaining how you arrived to ech answer for question 1

If you boil a protein in hydrochloric acid it will break down to its constituent amino...

If you boil a protein in hydrochloric acid it will break down to its constituent amino acids. Assume that you had done this to a typical protein and then had spotted these amino acids to a spot on the chromatograph. What pattern would you expect to see at the end of the experiment? Explain your prediction.

You have a mixture of the proteins listed below. Protein pI Molecular Weight (kDa) A 3.1...

You have a mixture of the proteins listed below. Protein pI Molecular Weight (kDa) A 3.1 265 B 6.9 93 C 10.3 96 D 7.1 43 E 8.6 189 1) You load the protein mix onto a cation exchange column at pH 5. Next, you apply a "washing" step by passing through buffer at pH 5. Finally, for your elution step, you apply a pH gradient starting from pH 2.0 to pH 13.0. (A gradient buffer system allows you to...

What do you think your 93rd amino acid is for this protein? the amino acid sequence...

What do you think your 93rd amino acid is for this protein? the amino acid sequence of the protein coded for by the wild-type TYRP1 is just below.

Even if you see ALL OF THE SUBJECTS in our theoretical blood glucose study have LOWER...

Even if you see ALL OF THE SUBJECTS in our theoretical blood glucose study have LOWER blood glucose after they take the medication, does that mean that the blood glucose worked? What other factors could be influencing a lower blood glucose among all the participants? Would those factors be confounders? Effect mediators? If you had to take into consideration all of those other factors, would you still do a t test?

You are attempting to purify a protein that has a pI of 9.0 from a mixture...

You are attempting to purify a protein that has a pI of 9.0 from a mixture of similarly sized proteins, and have access to different purification columns. For this protein, you would select as your first column for purification an: (a) Anion exchange (b) Cation exchange (c) Gel filtration (d) Heparin sulfate (e) Ammonium sulfate

You are provided with the amino acid sequence of an important human protein that is suspected...

You are provided with the amino acid sequence of an important human protein that is suspected to be membrane protein. How can you analyze the amino acid sequence to try to find out more information on the transmembrane nature of this protein and the region of the protein that is likely to be in the membrane?

You have to value a company based on its expected future cash flows. Interest rates are...

You have to value a company based on its expected future cash flows. Interest rates are currently 9%. If this company expects cash flow of $100000 next year (it is the end of year 0 or equivalently the beginning of the first year), growing at 7.8% each year until the end of the fifth year. Then, cash flow growth is expected to slow to 1.9 forever-after. What is the value of this company today, in thousands of dollars?

theoretical concept of walmart being based on its business strategy

Why is a protein more soluble when the pH is greater than or less than its pI?

Subjects