Question

Protein domain with zinc finger motifs are frequently used to interact with a specific sequence of DNA and found in many activators.

A.True

B.False

Answer 1

Answer-

According to the given question-

• Several techniques such as Genome editing and change in gene expression by using synthetic DNA binding are most frequently used today.
• Because they had developed some  DNA binding molecules which have very high binding specificity. such as TALE-domains, zinc fingers or ZFs, as well as the RNA component found in CRISPR/Cas9 system.
• These DNA binding modules are used by first linked with either (a) nucleases which are responsible for cutting DNA followed by induction of DNA repair processes, or (b) linked or fused with protein domains which are responsible for either activating  or repressing the gene transcription which are found near the targeted site in  genome.
• Zinc Fingers DNA binding domains or ZFDBDs are small in size and easily can  penetrate membrane of  cell and they do not need tag. so we can say that they do not need DNA or RNA intermediate for delivering inside the cell.
• ZF generally is made up of an α-helix and two β-sheets and interacts with 3 base pairs of DNA. Most prevalent DNA binding motif in eukaryotic transcription factors is C2H2-ZF .
• RNA polymerase III transcription factor such as TFIIIA contained 9 C2H2 ZFs which is in contact with internal control region and out of which 3 interacts with DNA which have CpG-rich  and regulate RNA polymerase II transcription.
• Zinc Finger Motif also found in  sequence-specific DNA-binding proteins such as CTCF, and ZiF268.They also mediates  protein-protein interactions.

So the given sentence is true.