Question

In: Chemistry

why is it important to force the trendline in the plot of absorbance vs concentration through...

why is it important to force the trendline in the plot of absorbance vs concentration through the origin? Would adding the point (0,0) have had the same effect? Why or why not?

how low of a concentration do you think this technique can reliably measure?

Solutions

Expert Solution


Related Solutions

why is the graph of the force vs position not uniform
why is the graph of the force vs position not uniform
You wish to construct an absorbance versus concentration (A vs. C) plot for a FD&C food dye. Suppose you have a stock solution that is 2.13 x 10-5 M in this dye.
  You wish to construct an absorbance versus concentration (A vs. C) plot for a FD&C food dye. Suppose you have a stock solution that is 2.13 x 10-5 M in this dye. Complete the following table (fill in the blanks) showing how the different calculations were performed Stock Concentration Volume of Stock (mL) Volume of Diluent (mL) Diluted Sample Concentration (M) 2.13 x 10-5 M 5.00 0.00   3.67   1.56 x 10-5 2.50 2.50 1.07 x 10-5 1.33...
Why is it important that all absorbance readings for the calibration curve be measured at the...
Why is it important that all absorbance readings for the calibration curve be measured at the same wavelength?
how would one set up an excel spreadsheet and create a plot for electric force vs...
how would one set up an excel spreadsheet and create a plot for electric force vs the product of the two charges.
what are the merits of normal absorbance to concentration calibration curve and log of absorbance versus...
what are the merits of normal absorbance to concentration calibration curve and log of absorbance versus log of concentration curve. Also state the comparison of the results obtained by those methods. which results are better.
what are the merits of normal absorbance to concentration calibration curve and log of absorbance versus...
what are the merits of normal absorbance to concentration calibration curve and log of absorbance versus log of concentration curve. Also state the comparison of the results obtained by those methods. which results are better.
Evan obtained the following absorbance data: Concentration (mM) 13,16.2,19.4,22.6,25.8 Absorbance: 0.095,0.143,0.205,0.241,0.312 A graph of this data...
Evan obtained the following absorbance data: Concentration (mM) 13,16.2,19.4,22.6,25.8 Absorbance: 0.095,0.143,0.205,0.241,0.312 A graph of this data revealed the line equation is y = 0.0166x – 0.1223 with an R2 =0.99234. A). Evan measures an unknown solution with an absorbance of 0.448. What is the concentration of the solution? B).Evan takes 1.0 mL of a solution and dilutes it to 50.0 mL. The absorbance is 0.192. What is the concentration of the original solution?
1. The observation that the absorbance is linearly proportional to the concentration of dye is a...
1. The observation that the absorbance is linearly proportional to the concentration of dye is a statement of which law? 2. For this question, note that you diluted your prepared drink mix 1:10 before you obtained absorbance values, and therefore your calculated concentration of each dye is 1/10 that of the prepared drink mix. You calculated that the concentration of yellow food dye in the cuvette is 0.0141 mM.  Given that the molecular weight of the yellow 5 dye is equal...
Concentration (mol/L) Absorbance Trial #1 Absorbance Trial #2 Absorbance Trial #3 Average 0.08 .165 .185 .182...
Concentration (mol/L) Absorbance Trial #1 Absorbance Trial #2 Absorbance Trial #3 Average 0.08 .165 .185 .182 .177 0.16 .321 .341 .342 .335 0.24 .467 .486 .518 .490 0.32 .617 .683 .674 .658 0.40 .752 .763 .763 .761 Unknown number .436 .450 .469 .452 1. Calculate the linear regression (best-fit line) equation of absorbance vs. concentration for the five standard CuSO4 solutions using Excel. Attach a graph showing the data and linear-regression equation for the standard solutions. The graph must show...
1. Why should someone measure absorbance at 340nm? What are the advantages/disadvantages of using 355nm vs...
1. Why should someone measure absorbance at 340nm? What are the advantages/disadvantages of using 355nm vs 340nm? NADH absorbance is highest at 250nm, what are 2 distinct reasons we are not looking at that wavelength? 2. One of the known inhibitors of hexokinase is 2-deoxy-D-glucose. Predict the type of inhibitor that 2-deoxy-D-glucose might be and explain your answer.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT