In: Chemistry
Concentration (mol/L) |
Absorbance Trial #1 |
Absorbance Trial #2 |
Absorbance Trial #3 |
Average |
0.08 |
.165 |
.185 |
.182 |
.177 |
0.16 |
.321 |
.341 |
.342 |
.335 |
0.24 |
.467 |
.486 |
.518 |
.490 |
0.32 |
.617 |
.683 |
.674 |
.658 |
0.40 |
.752 |
.763 |
.763 |
.761 |
Unknown number |
.436 |
.450 |
.469 |
.452 |
1. Calculate the linear regression (best-fit line) equation of absorbance vs. concentration for the five standard CuSO4 solutions using Excel. Attach a graph showing the data and linear-regression equation for the standard solutions. The graph must show the best-first and the equation.
Best-fit line equation: _________________________________________ R2=______________________
Attach graph here:
2. Indicate which physical property is the independent and dependent variable in your best fit equation.
3. Determine the concentration of the unknown CuSO4 solution using your graph. Explain how you made this determination.
4. Given that the cuvette that you used have a length path of 10 mm, calculate the molar absorptivity of copper (II) sulfate solution using the obtained Beer Law equation based on your data set (Hint: use the best fit equation and any data set).
5. Please explain in your own words what is the relationship between Concentration and Absorbance.
6. During the next lab we will be collecting Absorbance data to get information about the rate law for a reaction. In the experiment we will graph all our data using absorbance instead of concentration. What additional experimental step do we avoid by using absorbance instead of concentration? Can we do that? Do you think that the trends will be the same with concentration? Explain. (Hint: Your answer to question 7 may help. )