Question

The outline of an HPLC instrument

Answer 1

High Performance Liquid Chromatography

High Performance Liquid Chromatography is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

This involves a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas).

The mobile phase flows through the stationary phase and carries the components of the mixture with it.

HPLC is basically a highly improved form of column liquid chromatography.

forced through under high pressures Instead of a solvent being allowed to drip through a column under gravity,

It makes much faster than Chromatography

Type Of HPLC

Normal Phase HPLC, Reverse Phase HPLC: Ion-Exchange HPLC

Solvent Resorvoir :Mobile phase is usually containing mixture of polar and non-polar.solvent

Pump : used to generating of high pressures mobile phase

Sample Injector : The injector can be a single injection or an automated injection system. An injector for an HPLC system should provide injection of the liquid sample within the range of 0.1-100 mL under high pressure

Columns : Columns are stationary phase ,usually made of polished stainless steel, are between 50 and 300 mm long and have an internal diameter of between 2 and 5 mm.

The HPLC detector;

located at the end of the column detect the analytes as they elute from the chromatographic column. Commonly used detectors are fluorescence, UV-spectroscopy, LCMS, electrochemical detectors.