Question

In: Biology

To complete the table,below, consider the following elements of the system: [lacI promoter, lacI protein coding...

To complete the table,below, consider the following elements of the system:

[lacI promoter, lacI protein coding region, lacZ/Y promoter, lacZ protein coding for B-Galactotosidase, lacY protein coding for Permease, and operator]

Complete the table by using the pull down menu to select for “+” or “-“ symbols to show if β-Galactotosidase or Permease protein are produced in bacteria with the follow genotypes.

(for reference)

B-Galactotosidase (lacZ)

Permease (lacY)

No Lactose

Lactose Present

No Lactose

Lactose Present

lacPI+ lacI- lacPzya- lacO+ lacZ+ lacY+

lacPI+ lacI- lacPzya+ lacO+ lacZ- lacY+

lacPI+ lacIS lacPzya+ lacOC lacZ+ lacY+

lacPI+ lacI+ lacPzya+ lacO+ lacZ- lacY+

Solutions

Expert Solution

lac repressor inhibits the expression of the lac operon if the inducer is absent, lactose is an inducer of the lac operon when the lactose is present lac repressor is inhibited by lactose, so lac operon can be expressed.

lacPI+ - promoter of lac I gene.

lacI+ - functional lac I gene that codes for lac repressor.

lacPzya+ - promoter of lac operon.

lacO+ -lac operator.

lacZ+- codes for beta-galactosidase

lacY+ - codes for permease.

beta-galactosidase permease
lactose present lactose absent lactose present lactose absent
lacPI+ lacI- lacPzya- lacO+ lacZ+ lacY+ lac I gene is mutated so functional lac repressor is not produced, also the promoter of the lac operon is mutated so this lac operon will not be expressed in any conditions - lac I gene is mutated so functional lac repressor is not produced, also the promoter of the lac operon is mutated so this lac operon will not be expressed in any conditions - lac I gene is mutated so functional lac repressor is not produced, also the promoter of the lac operon is mutated so this lac operon will not be expressed in any conditions - lac I gene is mutated so functional lac repressor is not produced, also the promoter of the lac operon is mutated so this lac operon will not be expressed in any conditions -
lacPI+ lacI- lacPzya+ lacO+ lacZ- lacY+ lac repressor is mutated, so lac operon will be expressed even if lactose is absent, but lac Z gene is mutated so beta-galactosidase cannot be produced - lac repressor is mutated, so lac operon will be expressed even if lactose is absent,but lac Z gene is mutated so beta-galactosidase cannot be produced - lac repressor is mutated, so lac operon will be expressed even if lactose is absent, so permease is produced in the cell always, + lac repressor is mutated, so lac operon will be expressed even if lactose is absent,so permease is produced in the cell always, +
lacPI+ lacIS lacPzya+ lacOC lacZ+ lacY+ lac repressor is lac Is, it is super-repressor, which inhibits the lac operon even if inducer is present, but this operon has constitutive operator which cannot be inhibited by lac repressor so lac operon is expressed always + lac repressor is lac Is, it is super-repressor, which inhibits the lac operon even if inducer is present, but this operon has constitutive operator which cannot be inhibited by lac repressor so lac operon is expressed always + lac repressor is lac Is, it is super-repressor, which inhibits the lac operon even if inducer is present, but this operon has constitutive operator which cannot be inhibited by lac repressor so lac operon is expressed always + lac repressor is lac Is, it is super-repressor, which inhibits the lac operon even if inducer is present, but this operon has constitutive operator which cannot be inhibited by lac repressor so lac operon is expressed always +
lacPI+ lacI+ lacPzya+ lacO+ lacZ- lacY+ here regulatory elements are functional but beta-galactosidase is mutated so beta-galactosidase cannot be produced even if inducer is present - here regulatory elements are functional but beta-galactosidase is mutated so beta-galactosidase cannot be produced even if inducer is present - here regulatory elements are functional, so lac operon is expressed in the presence of inducer, so permease is produced when lactose is present + here regulatory elements are functional, so lac operon is expressed in the presence of inducer, so permease is produced when lactose is present and it cannot be produced in the absence of inducer -

gladiator answered 2 years ago
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