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Jacob and Monod proposed the operon model by analyzing mutations in the different components of the...

Jacob and Monod proposed the operon model by analyzing mutations in the different components of the lac operon. Below are the mutations.

Mutation

Genotype

Consequence

Defective repressor

I-

The repressor is not able to bind to the operator.

Super-repressor

IS

The repressor remains active and binds to the operator even in the presence of the inducer.

Constitutive Operator

OC

The operator sequence is altered such that the active repressor can't bind it.

Would anyone be able to help me fill the table below? Be sure to indicate the presence (+) or absence (-) of b-galactosidase and permease activity when mutations are present in different combinations of the E. coli chromosome and on the F' plasmid.

b-Galactosidase Activity (Z)

Permease Activity (Y)

Lactose

Lactose

Genotype

Present

Absent

Present

Absent

I- OC Z+ Y- A-

IS OC Z- Y+ A-

IS O+ Z- Y+ A-/F’ I- O+ Z+ Y- A+

I- OC Z+ Y- A+/F’ I- O+ Z- Y+ A-

I- O+ Z+ Y+ A+/F’ I- OC Z+ Y- A-

I+ OC Z+ Y- A+/F’ I+ O+ Z- Y+ A+

IS OC Z- Y+ A+/F’ I- OC Z+ Y- A-

IS O+ Z+ Y- A-/F’ I+ O+ Z- Y+ A+

Solutions

Expert Solution

Repressor works in a trans manner, it means that repressor produced in plasmid can bind to the lac operon of the genome.

The operator works in cis-manner, it means that if there is a mutation in one operator of genomic operon then it will affect the expression of plasmid operon.

If the merodiploid condition has IS and OC then OC will show its effect

If LacZ is mutated then b-Galactosidase will not show its activity irrespective of lactose presence of absence.

If LacY is mutated then Permease will not show its activity irrespective of lactose presence of absence.

b-Galactosidase Activity (Z)   
Permease Activity (Y)
Lactose present lactose absent Lactose present lactose absent
I- OC Z+ Y- A- + + - -
IS OC Z- Y+ A- - - + +
IS O+ Z- Y+ A-/F’ I- O+ Z+ Y- A+ - - - -
I- OC Z+ Y- A+/F’ I- O+ Z- Y+ A- + + + +
I- O+ Z+ Y+ A+/F’ I- OC Z+ Y- A + + + +
I+ OC Z+ Y- A+/F’ I+ O+ Z- Y+ A+ + + - +
IS OC Z- Y+ A+/F’ I- OC Z+ Y- A- + + + +
IS O+ Z+ Y- A-/F’ I+ O+ Z- Y+ A+ - - - -

Please write to me if you need more help.

gladiator answered 2 years ago
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