Question

In: Biology

What are the components/ steps of the genetic mapping in plants?

What are the components/ steps of the genetic mapping in plants?

Solutions

Expert Solution

1. Fragmentation of genome: Fragmentation of the genome into overlapping clones is usually done by using rare cutting enzymes so as to produce large DNA fragments.

2. Preparation of a large fragment library: The fragments thus generated are ligated to cloning vectors of high capacity like YAC.

3. Overlapping clone identification: Two strategies are used to identify overlapping clones: one is clone fingerprinting and the other is chromosome walking. In the clone fingerprinting method, overlapping clones are identified based on common patterns of restriction enzyme fragments or repeat content observed between clones. In chromosome walking, the overlapping clones are identified based on hybridization of two overlapping clones to a particular STS marker.

4. Preparation of STS content map: Arranging the overlapping fragments based on the presence or absence of a signal for a particular probe.

please do like the answer

gladiator answered 2 years ago
Next > < Previous

Related Solutions

Come up with an analogy comparing cytogenetic mapping, to genetic mapping, to DNA sequence mapping. Be...
Come up with an analogy comparing cytogenetic mapping, to genetic mapping, to DNA sequence mapping. Be able to describe it. Don't use a country map as an analogy. an analogy that compares cytogenetic mapping, genetic mapping and dna sequence
How can the concept of recombination frequency be used in genetic mapping?
How can the concept of recombination frequency be used in genetic mapping?
How can the concept of recombination frequency be used in genetic mapping?
How can the concept of recombination frequency be used in genetic mapping?
What are the two types of genetic SI(self-incompatibility) in plants?
What are the two types of genetic SI(self-incompatibility) in plants?
What is Genetic engineering, history, steps involved in it, scope and importance?
What is Genetic engineering, history, steps involved in it, scope and importance?
A management device that involves mapping out a sequence of programs or project steps is a....
A management device that involves mapping out a sequence of programs or project steps is a. performance evaluation review technique. b. management by objectives. c. critical path method. d. strategic analysis.
Genetic Mapping of Innate Immune Defects. Septic shock is a very dangerous medical condition that is...
Genetic Mapping of Innate Immune Defects. Septic shock is a very dangerous medical condition that is described in your textbook on page 135. It is essentially an acute hyper-activation of the macrophage innate immune response, resulting in systemic (instead of localized) production of cytokines, with deleterious effects. Normally, local cytokine production at an infection causes “leakiness” in blood vessels, so that leukocytes can exit and migrate to the infection site (erythrocytes also leak out in the process, leading to the...
Using the blanks provided, put the steps of involved in mapping a bacterial chromosome by conjugation...
Using the blanks provided, put the steps of involved in mapping a bacterial chromosome by conjugation in chronological order using the numbers 1-6 in the blanks provided. a. ___ ___ Cells that mated are checked for transfer of other genes from the Hfr to the F– strain. b. ___ ___ At regular intervals, conjugation is interrupted by shearing the mating bacteria away from each other. c. ___ ___ For each time point, cells that mated are selected—for instance, by requiring...
Two-Point Mapping The general strategy used to determine the genetic distance between linked genes is to:...
Two-Point Mapping The general strategy used to determine the genetic distance between linked genes is to: 1. Make all of the genes of interest heterozygous in an individual or strain 2. Examine the gametes produced in the heterozygous individual(s) Using our example above. You have two pure-breeding lines of plants, one with green seeds and short height (GG tt) the other with yellow seeds and tall height (gg TT). You cross the two strains to make the genes heterozygous. What...
1. What are the steps involved in the change management process or cycle, the components of...
1. What are the steps involved in the change management process or cycle, the components of a change management project plan (planning) and the procedures for embedding change? 2. What organisational barriers might need to be overcome and what strategies might be put in place to overcome resistance to change, embed it and support acceptance of change in an organisation? 3. Why is it important to have a good understanding of environmental legislation that affects your business or the industry...
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT