Question

what is the importance of LDH in the cell cycle (what does it do & why?)

Answer 1

LDH important for cell cycle because LDH
(lactate dehydrogenase) is encoded by two independent and
developmentally regulated genes LDH1 and LDH2. These genes and their products have been
implicated in the control of a metabolic flux during parasite differentiation. In order to
investigated the significance of LDH1 and LDH2 in this process, we generated stable transgenic
parasite lines in which the expression of these two expressed isoforms of ldh was knocked down in a
stage-specific manner.
These LDH knockdown parasites exhibited variable growth rates in either the tachyzoite or the
bradyzoite stage, as compared to the parentalparasites.
Their differentiation processes were impaired when the parasites were grown under in
vitro conditions.
In order to evaluate whether the lowered LDH gene expression affects parasite
differentiation, we investigated whether the ldh knockdown parasite lines could differentiate
and form cysts in vitro.
One of the othere reason of importance of ldh for cell cycle a mutation in the genes controlling the
production of lactate dehydrogenase will lead to a medical condition known as lactate dehydrogenase
deficiency. Depending on which gene carries the mutation, one of two types will occur- either lactate
dehydrogenase-1(A) deficiency or lactate dehydrogenase-2(B)
deficiency. Both of these conditions affect how the body breaks down sugars, primarily in certain muscle
cells. Lactate dehydrogenase-1 deficiency is caused by a mutation to the LDH1 gene, while lactate
dehydrogenase-2 deficiency is caused by a mutation to the LDH2 gene.

In the case of ldh-2 deficiency, mutations to the LDH2 gene result in the production of an abnormal
ldh-2 subunit that can`t bind to the other subunits to form the complete enzyme. As with the ldh-1
deficiency, this mutation reduces the overall effectiveness in the enzyme.