explain how a QIAprep spin miniprep purification column (( with silica gel functional group (silanol))) works...

explain how a QIAprep spin miniprep purification column (( with silica gel functional group (silanol))) works in regards to pH / pKa for binding and elution of DNA

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queen_honey_blossom answered 1 year ago

Explain the basis for the stacking gel portion of a polyacrylamide gel. Describe how it works...

Explain the basis for the stacking gel portion of a polyacrylamide gel. Describe how it works with respect to the loaded protein samples as well as the running buffer components.

Explain how polarity P' is related to retention of the sample analytes on the silica column...

Explain how polarity P' is related to retention of the sample analytes on the silica column for both the normal-phase and reverse-phase procedures.

Explain how gel electrophoresis of DNA samples works and why it was performed.

How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein purification techniques?

By gel electrophoresis, how do you know if your protein binded to the column? How would...

By gel electrophoresis, how do you know if your protein binded to the column? How would you modify the purification next time to make it more efficient?

How does size exclusion column work and what are the rate limiting factors in purification method?

Biochem Lab: Explain the basis for the stacking gel portion of a polyacrylamide gel. Describe how...

Biochem Lab: Explain the basis for the stacking gel portion of a polyacrylamide gel. Describe how it works with respect to the loaded protein samples as well as the running buffer components.

Explain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why...

Explain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?

Functional Group Identification: What are the functional groups (alkanes, alkenes, alcohols, aldehydes, ketones, phenol)? how is...

Functional Group Identification: What are the functional groups (alkanes, alkenes, alcohols, aldehydes, ketones, phenol)? how is reaction with Br2: (and how this can be used to test for alkanes, alkenes with and without light) Explain Ceric nitrate test (tests for alcohol functional group): turns red in presence of hydroxyl, turns green-brown in presence of phenol. FeCl3 test: phenols turn red-purple 2,4-DNP (tests for aldehydes and ketones): yellow-red = carbonyl present, but it does not help you decide between the aldehyde...

Why was carbon chosen as the atomic backbone of life? Define a functional group and explain...

*Why was carbon chosen as the atomic backbone of life? *Define a functional group and explain their importance to life. *Describe the complete structure, classification and functions of the carbohydrates. *List and describe in detail the 4 protein structures. *Define an enzyme and explain how they work and their importance to living things. *Compare the different types of triglycerides and indicate which are not healthy and those that are healthy. Also, indicate why some are and are not healthy choices....

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