What is the function of Histidine and Aspartic Acid in Chymotrypsin?

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Expert Solution

Not only Histidine and Aspartic Acid, there is a catalytic triad specifically with residue numbers in chymotripsin as serine 195, histidine 57, and aspartate 102. These residues are distributed throughout the 240 amino acid residues found in chymotrypsin, but are located in close proximity in the active three-dimensional conformation of the enzyme as shown in below figure. (This 3D model is reproduced from Reference text book - Lehninger, biochemistry, otherwise i can't build it, hence it is easy to understand pictorially than in words)

Chymotrypsin enzyme belongs to serine proteases family, mainly its function is to hydrolysis of peptides, the mechanism is shown below for clear understanding how it will be function. The peptide bond (-CO-NH-) split by this enzyme is 10¹⁰ times faster than without this enzyme.

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queen_honey_blossom answered 1 year ago

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