Amino acid analysis of a nonapeptide yielded the following: Gly2, Glu, Met2, Lys, Arg2, Thr (subscript...

Amino acid analysis of a nonapeptide yielded the following: Gly2, Glu, Met2, Lys, Arg2, Thr (subscript 2's indicate two glycines, two methionines, and two arginines). Reaction of the intact nonapeptide with Sanger's reagent yielded FDNB-Arg; incubation with trypsin yielded free Arg, free Glu, a tripeptide, and a tetrapeptide. Reaction of the tripeptide with Sanger's reagent yielded FDNB-Gly, and reaction of the tetrapeptide with Sanger's yielded FDNB-Thr. Incubation of the above tripeptide with carboxypeptidase (identifies the C-terminal amino acid) identified Arg. Incubation of the above tetrapeptide with carboxypeptidase identified Lys. Reaction of the intact nonapeptide with cyanogen bromide yielded a tetrapeptide, a tripeptide, and a dipeptide. The dipeptide contained Arg and Glu, and the tetrapeptide also contained an Arg. Given this information, what is the sequence of nonapeptide? Enter the answer using the one-letter code.

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queen_honey_blossom answered 1 year ago

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