Amino acid analysis of a nonapeptide yielded the following: Gly2, Glu, Met2, Lys, Arg2, Thr (subscript...

Amino acid analysis of a nonapeptide yielded the following: Gly2, Glu, Met2, Lys, Arg2, Thr (subscript 2's indicate two glycines, two methionines, and two arginines). Reaction of the intact nonapeptide with Sanger's reagent yielded FDNB-Arg; incubation with trypsin yielded free Arg, free Glu, a tripeptide, and a tetrapeptide. Reaction of the tripeptide with Sanger's reagent yielded FDNB-Gly, and reaction of the tetrapeptide with Sanger's yielded FDNB-Thr. Incubation of the above tripeptide with carboxypeptidase (identifies the C-terminal amino acid) identified Arg. Incubation of the above tetrapeptide with carboxypeptidase identified Lys. Reaction of the intact nonapeptide with cyanogen bromide yielded a tetrapeptide, a tripeptide, and a dipeptide. The dipeptide contained Arg and Glu, and the tetrapeptide also contained an Arg. Given this information, what is the sequence of nonapeptide? Enter the answer using the one-letter code.

Expert Solution

queen_honey_blossom answered 2 years ago

The amino acid sequence of human adrenocorticotropin, a polypeptide hormone, is: Ser-Tyr-Ser-Met-Glu-His-Glu-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-Asp-Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Glu-Pro-Leu-Glu-Phe a)What is the appr

The amino acid sequence of human adrenocorticotropin, a polypeptide hormone, is: Ser-Tyr-Ser-Met-Glu-His-Glu-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-Asp-Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Glu-Pro-Leu-Glu-Phe a)What is the approximate net charge of this molecule at pH 3? ph 7? ph 9? Assume that the pKs of the terminal -NH3+ and -COOH groups are 7.8 and 3.6, respectively.

which rptoleyic enzye would cleave this peptide into the most fragments? Asn-Met-Thr-Gln-Gly-Arg-Cys-Lys-Val-Asn-Thr-Phe-Val-His-Glu-Leu-Val-Asp-Val-Cys-Phe-Lys-Glu A. Trypsin B.Chymotrypsin C.Thermolysint...

which rptoleyic enzye would cleave this peptide into the most fragments? Asn-Met-Thr-Gln-Gly-Arg-Cys-Lys-Val-Asn-Thr-Phe-Val-His-Glu-Leu-Val-Asp-Val-Cys-Phe-Lys-Glu A. Trypsin B.Chymotrypsin C.Thermolysint D.they would all given an equal amou

What is the pI for Lys-Glu-Ser?

You have been provided a mixture containing four amino acids (Glu, His, Pro and Lys) to...

You have been provided a mixture containing four amino acids (Glu, His, Pro and Lys) to be separated by ion-exchange chromatography using a Dowex 50 cation-exchange, prepared in a loading buffer at pH 2.0. The following solutions are made available: The amino acid mixture in 0.04M citrate buffer at pH 2.0. 0.04M citrate buffer, pH 4.5 0.04M citrate buffer, pH 8.0 0.04M citrate buffer, pH 10.5 Is complete resolution of all amino acids achieved if the buffers are allowed to...

Consider the following peptide: Cys-Arg-Pro-Asp-Thr-Leu-His-Lys-Gln-Glu-His How is the peptide different at pH = 6 and pH...

Consider the following peptide: Cys-Arg-Pro-Asp-Thr-Leu-His-Lys-Gln-Glu-His How is the peptide different at pH = 6 and pH 8? Which of the two pHs would you use to run an ion exchange column, and would you choose anion or cation exchange?

Determine which of these four peptides is most likely to become a beta sheet. Met-Leu-Lys-Ala-Ser-Ala-Leu-Glu-Lys-Leu-Ser-Glu Ala-Glu-Met-Leu-Gln-Lys-Arg-Gly-Cys-Gly-Asp-Glu...

Determine which of these four peptides is most likely to become a beta sheet. Met-Leu-Lys-Ala-Ser-Ala-Leu-Glu-Lys-Leu-Ser-Glu Ala-Glu-Met-Leu-Gln-Lys-Arg-Gly-Cys-Gly-Asp-Glu Lys-Thr-Val-Ile-Trp-Pro-Phe-Tyr-Ile-Gln-Ile-Gly Arg-Ser-Tyr-Glu-Gly-Leu-Lys-Arg-Ile-Ala-Glu-Ser I would like help with the question but also I would like to know what makes a peptide more likely to form a beta sheet over and alpha helix and vice versa.

A peptide has the following sequence: A-K-R-H-G-E-E-R (Ala-Lys-Arg-His-Gly-Glu-Glu-Arg). What is the pI for this peptide? You...

A peptide has the following sequence: A-K-R-H-G-E-E-R (Ala-Lys-Arg-His-Gly-Glu-Glu-Arg). What is the pI for this peptide? You can use even integer approximations for pKa values: I know the answer is 9.5 but I need to know how to get to this answer.

Given the following sequence asp-cys-met-lys-ser-glu; what is the pI? net charge at pH 7.0 and pH...

Given the following sequence asp-cys-met-lys-ser-glu; what is the pI? net charge at pH 7.0 and pH 4.0?

\Match the following descriptions with the corresponding amino acids: This amino acid is responsible for the...

\Match the following descriptions with the corresponding amino acids: This amino acid is responsible for the strength of rhino horns and wood lignin due to the strong hydrophobic interactions between them. Subtle decreases in the serum pH lead to the protonation of this amino acid, which promotes O2 release from hemoglobin. This amino acid is the nucleophile chymotrypsin uses to attack peptide bonds. This amino acid disrupts α-helices because it makes the nearby protein backbone too flexible (and prevents proper...

Shown following is the first 30 amino acid (one-letter code) region of this putative amino acid...

Shown following is the first 30 amino acid (one-letter code) region of this putative amino acid sequence for p18: 1YFNPS 6TSDWPT 11LAPAN 16YTFLF 21FLARY 26WYINL30 A) Based on the partial amino acid sequence provided, do you think that it is more likely that this region of p18 associates with a cellular membrane or is exposed to the cytosol? B) Why or why not? C) Describe how you would experimentally demonstrate if p18 was associated with the membrane or the cytosol.

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