In: Chemistry
a.) Proteins A and B with molecular weight of 16,500 and 35,400 move 4.60 cm and 1.30 cm respectively, during electrophoresis and in SDS-polyacrylamide gel. What is the molecular weight of protein C that moves 2.80 cm under the same conditions in the same gel?
b.) The sedimentation coefficient of a DNA molecule is 22.0 S. A dilute solution of the DNA is spun in a centrifuge at 40,000 rpm starting from a distance of 6.0 cm from the axis of rotation. How far will the DNA move in 20 min?