Question

In: Chemistry

In all ligand titrations, the concentration of ligand is varied around the KD of the complex....

In all ligand titrations, the concentration of ligand is varied around the KD of the complex. In a stoichiometric titration, what is the concentration of receptor in relationship to KD? How does this differ from a binding titration experiment? Can you get a KD from a stoichiometric binding Titration? Why do the stoichiometric titration experiment this way, and what kind of information does it give you?

Solutions

Expert Solution

The KD of the complex is known as the equillibrium dissociation constant.The concentration of the unbound ligand at which the binding site is 50%.The smaller the dissociation constant, the more tightly bound the ligand is or we can say higher the affinity between ligand and protein.

The molecules with one binding site, the concentration of the complex [AB]= [A]0[B]/KD+[B] where A0 = Kd[AB]/[B]+[AB].For a simple bimolecular reaction, total bound A molecule to the Bfree is a hyperbola:

Fractional saturation of A=(Bfree)/[Kd+(Bfree)]

yes we can get a Kd from a stoichiometric binding titration,when one fix the stoichiometric binding site.The importance of binding is to measure interactions between two molecules, such as a protein binding another protein, a micromolecule or a nucleic acid. The reactions must be at equillibrium at the time of measurement,and the concentration of one reactant must be varied.


Related Solutions

1. What is Y in terms of ligand binding? What is the definition of Kd? How...
1. What is Y in terms of ligand binding? What is the definition of Kd? How are Y and Kd related? What is the meaning of affinity between a ligand and a protein? How is Kd related to affinity? 2. What do the binding curves of hemoglobin and myoglobin look like? Why are they different, what give hemoglobin’s curve a distinctive shape? How does a lower pH effect the hemoglobin binding curve?
1. What is Y in terms of ligand binding? What is the definition of Kd? How...
1. What is Y in terms of ligand binding? What is the definition of Kd? How are Y and Kd related? What is the meaning of affinity between a ligand and a protein? How is Kd related to affinity? 2. What do the binding curves of hemoglobin and myoglobin look like? Why are they different, what give hemoglobin’s curve a distinctive shape? How does a lower pH effect the hemoglobin binding curve?
Assume that the distribution coefficient (Kd) for Hg(II) is 104.9 (log Kd=4.9), the concentration of suspended...
Assume that the distribution coefficient (Kd) for Hg(II) is 104.9 (log Kd=4.9), the concentration of suspended sediments (Csed) is 10 mg L-1, the settling velocity (vs) of the silt-size particles is 4.17 cm hr-1, the area (A) of the water body is 50 km2 and the mean depth (H) is 6 m. What is the rate constant for removal of Hg(II) by scavenging, in units of per day?
what is the HCl concentration if 47.3 ml of 0.250 M NaIH is required to titrations...
what is the HCl concentration if 47.3 ml of 0.250 M NaIH is required to titrations a 30.0ml sample of the acid? NaOH is the other compound
Argentometric titrations can be used to determine the concentration of a number of halides (see Table...
Argentometric titrations can be used to determine the concentration of a number of halides (see Table 7-1 in the book). However, if the sample contains a mixture of halides they will precipitate in certain order. Considering that information, which of the following will preferentially form during a titration with AgNO3? A .Silver acetate (Ksp = 2.0 x 10-3) B. Silver bromide (Ksp = 5.3 x 10-13) C. Silver chloride (Ksp = 1.8 x 10-10) D. AgF (very soluble) E. Silver...
Draw an example of an octahedral complex that takes part in a ligand to metal charge...
Draw an example of an octahedral complex that takes part in a ligand to metal charge transfer.
1. Define the following terms: a. Complex Compound b. Complex ion c. Ligand d. Differentiate between...
1. Define the following terms: a. Complex Compound b. Complex ion c. Ligand d. Differentiate between a monodentate and a bidentate ligand e. Chelates
sketch a Job's method plot below for a complex where the ratio of ligand to metal...
sketch a Job's method plot below for a complex where the ratio of ligand to metal is 3:1.
Ligand X forms a complex with both cobalt and copper, each of which has a maximum...
Ligand X forms a complex with both cobalt and copper, each of which has a maximum absorbance at 510 nm and 645 nm, respectively. A 0.263-g sample containing cobalt and copper was dissolved and diluted to a volume of 100.0 mL. A solution containing ligand X was added to a 50.0 mL aliquot of the sample solution and diluted to a final volume of 100.0 mL. The measured absorbance of the unknown solution was 0.546 at 510 nm and 0.399...
So, I have some questions about the analytical chemistry experiment "Titrations based on complex formation." 1-...
So, I have some questions about the analytical chemistry experiment "Titrations based on complex formation." 1- Can you determine Mg by displacement or back titration? 2- You need to know the amount of EDTA added to solution in back titration method but you do not need to know the amount of MgEDTA added in displacement titration. Why? Explain by giving related reactions. 3- What is the minimum pH value needed for satisfactory titrations of Ca+2 and Mg+2 cations with EDTA?...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT