Question

Classification of Microbes: understand the purpose and general methodology behind each method: biochemical characterization, serology (agglutination, ELISA, Immunofluorescence), genetic methods (GC content, DNA fingerprinting, gene identification -by PCR, Southern blotting microarrays, FISH, qPCR, RT-PCR)

Answer 1

Biochemical characteristics is based on bacterial metabolic and respiratory activity which are different based on different species of bacteria hence we identify them based o their test result conclusion and classify them in different group

In serology test bacterial identification and classification are based on their antigen, antibody, reaction wich are reacting with that type of antigen and antibodies and hence agglutinate and we observe the result and conclude them based on their reactions type.

In genetic method bacteria can be classified according their genetic makeup which include their GC content which are differ in different bacterial species and also their genetic code, gene sequence, present enzymes, and the molecular weight of protein, DNA, RNA, amino acids etc are used for the differentiation of bacterial species and classification system it also use some bioinformatics techniques which identify by their sequence analysis which carried out by pcr ,rt pcr etc molecular method for identifying the bacteria and classified them in different species...