What is an endospore stain? Why is it medically relevant What is an electron microscope? What...

What is an endospore stain? Why is it medically relevant

What is an electron microscope? What are some differences as compared to a light-microscope?

Solutions

Expert Solution

Answer1: Endospores (seed-like form but it is not a true spore) are dormant, tough and non-reproductive structure produced by some bacteria (bacillus, clostridia) to prevent the loss of reproductive spores in adverse conditions (lack of water, oxygen, nutrients, favourable pH and temperature etc.) for extended period of time. The endospore stains are differential stain used to stain help in visualisation of bacteria. The endospore stain used for the differentiation of bacteria into endospore former and non-endospore former.

Answer2: Based on the source of light used for the visualisation of the objective microscope are of different types. Light microscope; in which normal light (sunlight) used as a light source for the visualization of object. In an electron microscope; a beam of electron used as a source for the visualization of objects.

Comparative of light and electron microscope

Characteristics	Light microscope	Electron microscope
Source of light	Normal light	Moving beam of electron
Lenses	Normal lens (1X,5X, 10X,40X and 100X)	Coiled shaped electromagnets in place of lenses.
Resolution	Less (200nm)	More (0.5nm)
Magnification	Less (maximum 1500X)	High (100000X)
Colour	Colour micrograph can be seen	Black and white micrograph only saw
Type of specimen can be seen	Small organisms, invertebrates and even a whole cell can be seen	Cell organelles, smallest bacteria and viruses can be seen

gladiator answered 1 year ago

Next > < Previous

Related Solutions

What is the difference between A. Scanning Electron Microscope (SEM) B. Transmission Electron Microscope (TEM) C....

What is the difference between A. Scanning Electron Microscope (SEM) B. Transmission Electron Microscope (TEM) C. Atomic Force Microscope (AFM). The difference must include the Principle, Operation, and recent Applications

A Transmission Electron Microscope (TEM) is a kind of electron microscope that images by detecting electrons...

A Transmission Electron Microscope (TEM) is a kind of electron microscope that images by detecting electrons that pass through a sample (hence, the tranmission). This requires both very thin samples and much higher electron kinetic energies, at least an order of magnitude greater than a conventional Scanning Electron Microscope (SEM). If a particular TEM operates with an accelerating voltage of 150 kV, and therefore electrons with kinetic energies of 150 keV (recall KE = q∆V ), calculate the theoretical smallest...

Acid Fast & Endospore Stain: 1. Does the presence of an acid-fast organism in a clinical...

Acid Fast & Endospore Stain: 1. Does the presence of an acid-fast organism in a clinical specimen always suggest serious clinical disease? 2. To check for active tuberculosis, what type of clinical specimen should be taken from a patient? 3. Why is it important to know whether or not bacterial cells form endospores?

Describe a spore forming bacterium stained with the endospore stain and magnified 1000X. Describe the spore...

Describe a spore forming bacterium stained with the endospore stain and magnified 1000X. Describe the spore and vegetative cell and note the colors you would expect to see using the stains we performed. What would you expect the Gram reaction of this cell to be?

What is the major advantage of electron microscopes over light microscopes? What type of electron microscope...

What is the major advantage of electron microscopes over light microscopes? What type of electron microscope would be used to view the three-dimensional features of a cell?

1) You are performing an endospore stain of a 72 hour Clostridium sporogenes culture. You forget...

1) You are performing an endospore stain of a 72 hour Clostridium sporogenes culture. You forget to turn on the heat on your water beaker. What will you see under the microscope: a) Spores will be green b)Everything will be green c) vegetative cells will be pink e) everything will be pink 2) You are performing an acid-fast stain of Mycobacterium smegmatis and forget to use the acid alcohol. WHat color will the cells appear under the microscope? a)green or...

You're evaluating a new electron microscope for the QA (quality assurance) unit. The microscope will cost...

You're evaluating a new electron microscope for the QA (quality assurance) unit. The microscope will cost $19,000 to buy and another $2,000 to install, and will be sold for $1,800 after 3 years. It falls into the 3-year MACRS class, with depreciation rates as follows: Year 1 2 3 4 Depreciation rate 33% 45% 15% 7% The microscope will require an inventory of spare parts worth $5,000. The equipment will not increase revenue, but will save the company $10,000 in...

Explain in your own words the working of scanning electron microscope and Atomic Force microscope. In...

Explain in your own words the working of scanning electron microscope and Atomic Force microscope. In two paragraphs one for each. Include references if used.

Intro You're evaluating a new electron microscope for the QA (quality assurance) unit. The microscope will...

Intro You're evaluating a new electron microscope for the QA (quality assurance) unit. The microscope will cost $24,000 to buy and another $2,000 to install, and will be sold for $1,800 after 3 years. It falls into the 3-year MACRS class, with depreciation rates as follows: Year 1 2 3 4 Depreciation rate 33% 45% 15% 7% The microscope will require an inventory of spare parts worth $5,000. The equipment will not increase revenue, but will save the company $12,000...

What is endospore-forming cell?

Subjects