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What is an endospore stain? Why is it medically relevant What is an electron microscope? What...

  1. What is an endospore stain? Why is it medically relevant
  1. What is an electron microscope? What are some differences as compared to a light-microscope?

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Expert Solution

Answer1: Endospores (seed-like form but it is not a true spore) are dormant, tough and non-reproductive structure produced by some bacteria (bacillus, clostridia) to prevent the loss of reproductive spores in adverse conditions (lack of water, oxygen, nutrients, favourable pH and temperature etc.) for extended period of time. The endospore stains are differential stain used to stain help in visualisation of bacteria. The endospore stain used for the differentiation of bacteria into endospore former and non-endospore former.

Answer2: Based on the source of light used for the visualisation of the objective microscope are of different types. Light microscope; in which normal light (sunlight) used as a light source for the visualization of object. In an electron microscope; a beam of electron used as a source for the visualization of objects.

Comparative of light and electron microscope

Characteristics

Light microscope

Electron microscope

Source of light

Normal light

Moving beam of electron

Lenses

Normal lens (1X,5X, 10X,40X and 100X)

Coiled shaped electromagnets in place of lenses.

Resolution

Less (200nm)

More (0.5nm)

Magnification

Less (maximum 1500X)

High (100000X)

Colour

Colour micrograph can be seen

Black and white micrograph only saw

Type of specimen can be seen

Small organisms, invertebrates and even a whole cell can be seen

Cell organelles, smallest bacteria and viruses can be seen


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What is endospore-forming cell?
What is endospore-forming cell?
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