According to your study, when is the best time to collect purified Con A product? A,...

According to your study, when is the best time to collect purified Con A product?

A, During the wash.

B, Immediately after adding the elution buffer.

C, Allow the charged column to soak in the eluent for a while, then collect the inital drops immediately after uncapping.

D, After uncapping, skip some drops, than collect the elutant.

Solutions

Expert Solution

•Column chromatography is a precursory technique used in the purification of compounds based on their hydrophobicity or polarity. The main principle involved in column chromatography is adsorption of the solutes of a solution through a stationary phase and separates the mixture into individual components. This is based on the affinity towards the mobile phase and stationary phase.

•After addition of compound of our interest, settle down the column first then add the elusion buffer.

•Uncap the tap and start to collect the fraction of elutants .

•First small fraction of elusion buffer contain the impurities of gel [mostly], so throw off these few drops and then collect the coming elutant which having pure product. [here Con A].

•

•So your answer must be

•D, After uncapping, skip some drops, than collect the elutant.

gladiator answered 1 year ago

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