In newly formed neural tube, the intermediate region close to
the sulcus limitans, has been not exposed to molecules that induce
ventral or dorsal characteristics. You perform explant experiments
in which you take intermediate neural tube (host tissue) and
culture them with donor tissue explants that induce specific spinal
cord markers in the host explant.
1. The induced host tissue express Shh following transplant.
a. What donor tissue have you explanted next to the host tissue,
what signal is released...