What is the advantage of UV/VIS double-beam instruments over
UV/VIS single-beam instruments? What is the advantage of UV/VIS
double-beam-in-time instruments over UV/VIS double-beam-in-space
instruments?
on your own words explain reaction and influence in the UV-vis
spectrum
how does benzonphenone affects the spectrum of ethanol and why?
(benzonphenone in ethanol)
how does benzonphenone affects the spectrum of cyclohexane and
why? (benzonphenone in ethanol)
how does benzonphenone affects the spectrum of n-hexane and why?
(benzonphenone in n-hexane )
how does benzonphenone affects the spectrum of methylene
chloride and why? (benzonphenone in methylene chloride)
What is the difference between UV-vis fluorescence and UV
absorption (please be detailed with your response and explain the
correlation between the two as well as their distinct
properties)?
What does the “vertical transition” in electronic spectroscopy
(UV-Vis) mean and what is the
rationale for this approximation? Please explain and show all
work.
Beer-Lambert law relates the attenuation of light to the
properties of the samples in UV-Vis spectrometer; however, in
general, the experimental results do not follow the Beer’s law.
What are the limitations of Beer’s law?
There are several distinct differences between IR and UV-Vis
spectroscopy. Discuss the following as they relate to:
(a) The types of interactions responsible for the
signals generated
(b) Source of radiant energy
(c) Signal detection
(d) Sample cells
(e) Sample preparation
1. Which is better for identification, IR or UV/Vis
spectrophotometry and why?
2. Why does a UV/Vis absorbance spectrum have discreet “bumps”
or “absorbance bands”?
3. Why does the absorbance spectrum for most molecules in
solution look very smoothed out, i.e. why doesn’t it have sharp
bumps like those observed in the gas phase?
Tell how UV-vis spectrometry can be used to differentiate the
following compounds. Calculate approximate ymax values for each
compound when you can.
a) 1,4 hexadiene, 2,4-hexadiene, and 1,3 hexadiene
b) cyclohexanone, 2-cyclohexenone, and 2,4-cyclohexadienone