Why is SDS-PAGE well suited in studies of cyclin modification but not in studies of retinoblastoma...

Why is SDS-PAGE well suited in studies of cyclin modification but not in studies of retinoblastoma pRb phosphorylation?

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SDS-PAGE is basically denaturing gel and the cyclin modifications are the addition of some additional proteins to it in addition to the phosphorylation of cyclin, but the modification of the pRb is only phosphorylation and this phosphorylation removes Rb inhibiting activity on E2F. Hence when run on SDS-PAGE gel, for cyclin protein additional proteins also are present on the gel which tell us that the cyclin has been modified but the Rb both in active and inactive form has little difference when run on an SDS-PAGE gel. Hence the SDS-PAGE is well suited in studies of cyclin modification but not in studies of retinoblastoma pRb phosphorylation.

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gladiator answered 1 year ago

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