Question

In: Biology

What are some materials needed to test for pesticides in baby food?

What are some materials needed to test for pesticides in baby food?

Solutions

Expert Solution

Answer- Test for pesticides are as follows-

Baby food samples were tested for pesticides using standard procedures employed by the Food and Drug Administration (FDA) and described in the FDA Pesticide Analytical Manual. The Luke extraction method was used for all polar and non-polar pesticides detectable by either Gas chromatography (GC) or High Performance Liquid Chromotography (HPLC). Materials required in LUKE method are as follows-

Apparatus-

  • Automatic pipettes, suitable for handling volumes of 10 µL to 5000 µL and 1 mL to 3 mL
  • 250 ml PTFE centrifuge tubes
  • 40 ml glass tubes with caps
  • Vortex
  • Turrax homogeniser
  • Centrifuge, suitable for the centrifuge tubes employed in the
    procedure and capable of achieving at least 3300 rpm
  • Water bath
  • Injection vials, 2 ml, suitable for LC and GC auto-sampler
  • Volumetric flasks

4. Chemicals-

  • Acetone p.a.
  • Petroleumether
  • Dichloromethane
  • Anhydroussodium sulphate
  • Ammonium formiate
  • Ultra-purewater
  • MethanolHPLC grade
  • Isooctane
  • Toluene
  • Acetic acid
  • Pesticides standards.

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