Question

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The following must contain the results/answers to the questions below for the bacteria: Enterobacter cloacae

Please answer the following questions A-O for Enterobacter cloacae

A. Gram Stain

B. Shape

C. Arrangement

D. Fermentation on lactose

E. Fermentation on glucose

F. Fermentation on mannitol

G. Fermentation on sucrose

H. EMB

I. Mannitol Salt Agar

J. Growth on MacConkey

K. PEA (Phenyl Ethyl Alcohol Agar)

L. Citrate utilization

M. Catalase

N. Gas production

O. Aerotolerance

Answer 1

A. Gram stain of Enterobacter cloacae :-

cloacae is frequently grown at 30 °C on nutrient agar or broth or at 35 °C in tryptic soy broth. It is a rod-shaped, Gram-negative bacterium, is facultatively anaerobic, and bears peritrichous flagella.

B. Shape of

  Enterobacter cloacae is a rod-shaped, gram-negative bacteria from the Enterobacteriaceae family.

C. Arrangement of

Enterobacter cloacae lives in the mesophilic environment with its optimal temperature at 37 °C and uses its peritrichous flagella for movement.

D. Fermentation on lactose

Lactose usually is fermented rapidly by Escherichia, Klebsiella and some Enterobacter species and more slowly by Citrobacter and some Serratia species. Proteus, unlike the coliforms, deaminates phenylalanine to phenylpyruvic acid, and it does not ferment lactose.

E. Fermentation on glucose

Enterobacteriaceae are gram-negative, non–spore-forming, facultative anaerobes that ferment glucose and other sugars, reduce nitrate to nitrite, and produce catalase but (with the exception of Plesiomonas) do not produce oxidase.

F. Fermentation on mannitol

Positive

G. Fermentation on sucrose :-

Positive

H. EMB :-

Only gram-negative bacteria grow on EMB agar. Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar. Based on a moderate rate of lactose fermentation and acid production, Enterobacter species produces large, mucoid, pink to purple colonies with no metallic green sheen on EMB agar.

I. Mannitol salt agar :-

Positive

J. Growth on MacConkey :-

  On MacConkey agar, E. cloacae and E. aerogenes commonly appear as pink

K. PEA :-

Negative

L. Citrate utilization :-

  Enterobacter species are able to utilize sodium citrate as the sole carbon source .

M. Catalase :-

  They are catalase positive

N. Gas production :-

  They ferment glucose and lactose with the production of acid and gas.

O. Aerotolerance :-

Positive