For the following scenarios involving HPLC, state whether the internal standard method, or the standard addition...

For the following scenarios involving HPLC, state whether the internal standard method, or the standard addition method would be more appropriate and why.

1)You use a multi-step method (including extraction steps) to prepare your sample prior to analysis.

2)You are analysing a sampleof industrial waste. It is a very complex sample which you know will contain many unknown impurities.

3)Yourdetector response varies slightly with temperature, however yourinstrument is not temperature controlled.

Expert Solution

In the following Scenarios given in the question does not favours the internal standard method and standard addition method involving HPLC because in internal standard method it might improve the data quality but it is used to determine the concentration of analytes using response factor it have similar retention time and the similar derivitization also it might be stable but does not interfere with the sample components.

On the other hand in standard addition method it required matrix effect .i.e. sample matrix also contributes to the analytical signals and it is impossible to measure sample and standard using traditional calibration curve method.

queen_honey_blossom answered 2 years ago

How can you combine standard addition and internal standard techniques in an HPLC experiment?

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