Mass spectrometry is an extremely versatile detection system for gas chromatography. Describe the major reasons why it is more difficult to co ...

Mass spectrometry is an extremely versatile detection system for gas chromatography. Describe the major reasons why it is more difficult to combine HPLC with mass spectrometry than it is to combine GC with mass spectrometry.

Expert Solution

The basic requirement in mass spectrometry (MS) is a gas-phase sample. Solute dissolved in a solvent is the output of the high-performance liquid chromatography column (HPLC) whereas gas is the output of the gas chromatography (GC) column. Thus, high-performance liquid chromatography is not compatible with mass spectrometry.

Also we observed that, the solvent is first vaporized in liquid chromatography/mass spectrometry (LC/MS) to produce gas. Its volume is 10 to 1000 times greater than the carrier gas in gas chromatography. This is indicates that most of the solvent in LC/MS has to be removed.

Therefore, HPLC combines with mass spectrometry in a quite difficult manner than GC.

Nabeel answered 1 year ago

Why are carboxylic acids, ketones and esters suitable compounds for analysis by gas chromatography-mass spectrometry (GCMS)

Gas Chromatography- Mass Spectrometry If the chromatographic elution peaks in the sample are far apart and...

Gas Chromatography- Mass Spectrometry If the chromatographic elution peaks in the sample are far apart and analysis takes a relatively lon time, should you increase or decrease the column temperature program to improve analysis time? Explain your reasoning.

Why is tandem mass spectrometry more useful in analysis of peptides and proteins?

List and explain two reasons why the fractional reserve system makes it difficult for the Federal...

List and explain two reasons why the fractional reserve system makes it difficult for the Federal Reserve Bank to control the exact size of the money supply. What, if anything, can the Fed do to keep these problems in check?

Gas chromatography questions 1. why is GC separation more efficient than a fractional distillation? 2....

Gas chromatography questions 1. why is GC separation more efficient than a fractional distillation? 2. what characteristics must the liquid stationary phase have? 3. how do the flow rate of the carrier gad and the column temperature affect the retention time of a compound on a GC column? 4. Describe a method for identifying a compound using GC analysis

a, Describe six major reasons for accounting diversity: legal system, taxation, providers of financing, inflation, political...

a, Describe six major reasons for accounting diversity: legal system, taxation, providers of financing, inflation, political and economic ties, and culture. b. Describe which factor or factors appear to have exerted influence on the development of accounting in China, Japan, Germany, Mexico and United Kingdom? c. Identify the distinguishing features of the accounting system in each of these countries.

Describe two major reasons why fluorescence spectrophotometry is a very sensitive analytical technique? (hint, one of...

Describe two major reasons why fluorescence spectrophotometry is a very sensitive analytical technique? (hint, one of the reasons involves the equation I = KP0C) If you wish to analyze a molecule using fluorescence spectrophotometry but that molecule does not fluoresce, what can you do? What characteristics of a molecule make it likely to fluoresce?

describe atleast two reasons why a perpetual inventory system might be considered preferable to a periodic...

describe atleast two reasons why a perpetual inventory system might be considered preferable to a periodic inventory system. explain in detail

Describe the meaning of “System Sustainment” why this phase cost more than other phases in the...

Describe the meaning of “System Sustainment” why this phase cost more than other phases in the system development process?

Question