Question

Exon Junction Complex (EJC) has 4 components: elF4AIII, MAGOH, Y14, and MLN51.

What is the PDB code(that can be used in Chimera) that represents all these four components? What is their relationship and function.

Answer 1

COMPONENT	PDB CODE
elF4AIII	2VSO
MAGOH	2HYI
Y14	1RK8
MLN51	2J0S

elF4AIII

• The eukaryptic initiation factor-4A family consists of 3 closely related proteins elF4AI, elF4AII, elF4AIII.
• These factors are required for the binding of mRNA to 40S ribosomal subunits.
• In addition these proteins are helicases that function to unwind double stranded RNA.
• It has implications in the normal translation as well as transformation and progression of cancerous cells.
• elF4AIII is located on chromosome no.17 q25.3

MAGOH

• It is a protein that is encoded by the MAGOH gene.
• Drosophila that have mutations in their mago nashi gene produce progeny with defects in germplasm assembly and germline development.
• This gene encodes the mammalian mago nashi homolog.
• In mammals, mRNA expression is not limited to the germplasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts.

Y14

• The EJC core protein, Y14, functions with its partners in nonsense-mediated mRNA decay and translation enhancement.

MLN51

• The RNA binding protein metastatic lymph node 51 (MLN51) is a translation enhancer.
• Overexpression of MLN51 preferentially increased the translation of intron-containing reporters via the EJC, whereas silencing of MLN51 decreased translation.
• Modulation of the MLN51 level in cell-free translational extracts confirmed its direct role in protein synthesis.