Question

Premise, You have two clear glasses of colored liquid, both are green. One glass has more condentrated dye then the other glass does( so one glass has more water-dye ratio, the other has more dye-water ratio) why does the more concentrated glass of colored liqued have a higher absorbance level then other glass?

Answer 1

According to Beer Lambert's law, the absorbance level or absorbance capability of a solution is determined using the following equation:

A = e*l*C

Here 'e' is the molar absorptivity of the analyte. In this case, the analyte is the green colored dye.

'l' is the cuvette length which is kept constant equal to 1 cm in spectrophotometric readings.

'C' is the conc. of the analyte solution whose absorbance level is to be measured.

As we can see, A is directly proportional to C.

So, the greater the conc. of the analyte solution, the greater is its absorbance level.

Since the solution with higher dye:water ratio tends to be more concentrated, so it will have higher absorbance level than the other glass.