Question

Which test uses sodium hydroxide and copper sulfate? What does it test for? Is there a color change? Does it need to be heated?

		Benedicts Sugar Yes, it turns to a copper red color Yes, it needs to be heated
		Biuret Protein Yes, it turns purple to violet No, it does not need to be heated
		Specific gravity Density No, it does not change color No, it does not need to be heated
		Diastik Sugar Yes, the color change indicates the level present No, it does not need to be heated

Which test would be used to measure patient hydration and kidney functions (ex. stenosis of renal artery or glomerulonephritis)?

		Biuret Protein Yes, it turns purple to violet No, it does not need to be heated
		Specific gravity Density No, it does not change color No, it does not need to be heated
		Benedicts Sugar Yes, it turns to a copper red color Yes, it needs to be heated
		Diastik Sugar Yes, the color change indicates the level present No, it does not need to be heated

Which test uses sodium citrate and sodium carbonate? What does it test for? Is there a color change? Does it need to be heated?

		Diastik Sugar Yes, the color change indicates the level present No, it does not need to be heated
		Specific gravity Density No, it does not change color No, it does not need to be heated
		Benedicts Sugar Yes, it turns to a copper red color Yes, it needs to be heated
		Biuret Protein Yes, it turns purple to violet No, it does not need to be heated

Answer 1

Ans-1. Biuret

Protein

Yes, it turns to purple to violet

No, it does not need to be heated

Biuret test is used for the test of proteins . The test is done by putting food sample into the test tube. Adding water and stirring it. Then add equal volume of pottasium hydroxide solution to the tube and stir it . Add two drops of copper sulphate solution and stir. Record the colour of the solution. Biuret reagent is blue or purple in colour which changes to violet. There was no need of heating in the procedure.

Ans-2. Benedict's

Sugar

Yes, it turns to copper red colour

Yes, it requires heating

Benedict's solution is a clear blue solution composed of copper sulphate, sodium citrate and sodium carbonate. It is used for the test of sugars.

The procedure is take approx 1 ml of sample in a test tube. 2 ml or 10 drops of Benedict's reagent us added . The solution is heated now in a boiling water bath for 3-5 min. Observe the change in colour. The colour changes from brick red to rusty red or copper red depending on the amount of sugar. And heating is required in the procedure.