Question

Which phase carries the analyte through the Chromatographic separation?

Standard Phase, Detection Phase ,Injection Phase, Stationary Phase, Mobile Phase

Which phase slows the analyte down in the Chromatographic separation?

Standard Phase, Detection Phase ,Injection Phase, Stationary Phase , Mobile Phase

If you increase the flow rate of the mobile phase, will the analyte come out ________?

Slower, In two Peaks, Faster, Unchanged

In this separation the stationary phase is polar and the mobile phase is non-polar. Youre separating two molecules, one polar and one non-polar. Which molecule comes out first? Non-polar, No Difference, Polar

Answer 1

in chromatogrphy have mainly two phases.stationary phase and mobile phase. for example silica is stationary phase and he came is mobile phase.when sample is loaded in column the compounds thave different attractive force on stationary phase. The compound with less interaction with stationary phase come out with the mobile phase first.

Standard phase have interaction with analyte. So if it have higher interaction it slow down the movement of analyte with mobile phase..

If we increase the flow rate of mobile phase the analyte coming out also increases. Beacuse the analyte is coming with mobile phase.if mobile phase coming fast the analyte also come fastly

Stationary phase is polar. So it have more interaction with the analyte is polar.so stationary phase hold the polar analyte.the mobile phase is non polar.so it have more interaction with non polar analyte.so no(n polar analyte will come first