Look up information for the ladder you will be using (Lambda-Phage viral DNA digested with HinDIII)....

Look up information for the ladder you will be using (Lambda-Phage viral DNA digested with HinDIII). Save this information and also take a snapshot of the ladder (include here) with corresponding basepairs. This ladder would have been/was prepared for you.

Be observant of the band distribution (are they evenly spaced? are their distinctive bands? any close together? These observations will be important for identifying the size of the bands YOU will see on your own gel

Expert Solution

The above is the bad pattern observed when digesting lamda DNA with Hind - III. The digestion with HInd-III gives rise to number of fragments of the DNA of varying sizes. When these restriction fragments are run through the gel, based on the size of the fragments, they travel at different rates. This results in formation of bands at different regions on the gel. Larger fragments move slowly, so we see the larger fragments near to the region of loading. Smaller fragments move faster and are more near to the positive electrode.

Bands are not evenly distributed. Bands move based on their size, that is why their distribution also is not even. Larger bands with 23130 basepairs are near to the region of loading. Band with 564 base pairs is more near to the positive electrode as it travels faster.

Using the above ladder the size (bps) of the unknown DNA can be understood,

gladiator answered 2 years ago

Topic: Sizing DNA fragments via standard curves using horizontal electrophoresis Between a low quality ladder (DNA...

Topic: Sizing DNA fragments via standard curves using horizontal electrophoresis Between a low quality ladder (DNA marker) and high quality ladder (Kb ladder), which one produces more accurate estimates on a standard curve? Why?

In phage display using a phagemid vector derived from M13 filamentous phage, you need to transfect...

In phage display using a phagemid vector derived from M13 filamentous phage, you need to transfect a helper phage to propagate the phagemid vector. Explain why ?

why should you look up the density of an organic solvent before using it in an...

why should you look up the density of an organic solvent before using it in an extraction? What is meant by the term 'Immiscible" and why is this factor important when performining an extraction?

You are setting up a PCR reaction using DNA from a bacteria with a genome size...

You are setting up a PCR reaction using DNA from a bacteria with a genome size of 17 megabase-pairs (mb). How many nanograms of DNA will you need to add to the reaction to ensure there are at least 100 copies of the single gene you are trying to amplify? (assume the average molecular weight of a nucleotide pair is 650). (2 pt)

Look up information from the ENRON Case and discuss it in detail.

Problem 2. Using Wikipedia, look up the entry for Sarbanes-Oxley Act. Look over the table of...

Problem 2. Using Wikipedia, look up the entry for Sarbanes-Oxley Act. Look over the table of contents and find the section that describes Section 404 and give it a read. Based on your textbook reading and Wikipedia review, briefly respond to the following. What does Section 404 require of management’s internal control report?

Question 22 options: Look up the requested values using book tables. You may check with a...

Question 22 options: Look up the requested values using book tables. You may check with a calculator, but enter the table value. Let T denote a t-distribution variable with 10 degrees of freedom. Find: P(T<1.372)=?

1-Look up the requested values using book tables. You may check with a calculator, but enter...

1-Look up the requested values using book tables. You may check with a calculator, but enter the table value. Let T denote a t-distribution variable with 14 degrees of freedom. Find: P(T>1.761)=? Note: Enter X.XX AT LEAST ONE DIGIT BEFORE THE DECIMAL, TWO AFTER and round up. Thus, 27 is entered as 27.00, 3.5 is entered as 3.50, 0.3750 is entered as 0.38 2-Look up the requested values using book tables. You may check with a calculator, but enter...

1. One way of accessing financial information about a company is to look it up at...

1. One way of accessing financial information about a company is to look it up at the company's website. Access the most recent annual report of a company of your choice from the company's website and address the following points. a. Review the company's footnotes (notes accompanying the financial statements) to discover how it applies the revenue recognition principle and when it recognizes revenue. Report what you discover. b. Based on your observation of the financial statements, what amount should...

Using Java, Explain how to implement a functional interface using a lambda expression. You may include...

Using Java, Explain how to implement a functional interface using a lambda expression. You may include small sections of code to illustrate your explanation.

Question