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In: Biology

Need a precise rundown of differences between the cell envelope of a Gram-negative bacterial cell and...

Need a precise rundown of differences between the cell envelope of a Gram-negative bacterial cell and a Gram-positive bacterial cell, how the cell membranes and cell walls are different between them and important features.

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Expert Solution

Gram-negative Bacteria Gram-positive Bacteria
Gram reaction Can be decolourized to accept counter stain (Safranin or Fuchsine); stain red or pink, they don't retain the Gram stain when washed with absolute alcohol and acetone. Retain crystal violet dye and stain dark violet or purple, they remain coloured blue or purple with gram stain when washed with absolute alcohol and water.
Peptidoglycan layer Thin (single-layered) Thick (multilayered)
Teichoic acids Absent Present in many
Periplasmic space present Absent
Outer membrane Present Absent
Lipopolysaccharide (LPS) content High Virtually none
Lipid and lipoprotein content High (due to presence of outer membrane) Low (acid-fast bacteria have lipids linked to peptidoglycan)
Flagellar structure 4 rings in basal body 2 rings in basal body
Toxins produced Primarily Endotoxins Primarily Exotoxins
Resistance to physical disruption Low High
Inhibition by basic dyes Low High
Susceptibility to anionic detergents Low High
Resistance to sodium azide Low High
Resistance to drying Low High
Cell wall composition The cell wall is 70-120 Å (ångström) thick; two layered. Lipid content is 20-30% (high), Murein content is 10-20% (low). The cell wall is 100-120 Å thick; single layered. Lipid content of the cell wall is low , whereas Murein content is 70-80% (higher).
Mesosome Mesosome is less prominent. Mesosome is more prominent.
Antibiotic Resistance More resistant to antibiotics. More susceptible to antibiotics

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