What environmental factor was required for the pGLO plasmid to express GFP?

Expert Solution

gladiator answered 2 years ago

1) The pGLO plasmid does not have EcoR1 sites flanking the GFP/araC region. Why is this...

1) The pGLO plasmid does not have EcoR1 sites flanking the GFP/araC region. Why is this an issue and how would it be dealt with? 2) Explain why it was necessary to "clean up" PCR products before performing restriction digestion. Predict what would have happened if we skipped this step.

The AmpR gene on plasmid pGLO gives cell resistance to Ampicillin. Explain what that means and...

The AmpR gene on plasmid pGLO gives cell resistance to Ampicillin. Explain what that means and how this gene provides that.

after transforming E colo with the pGLO plasmid, you incubate the vials with LB broth for...

after transforming E colo with the pGLO plasmid, you incubate the vials with LB broth for 30 min first. Explain what may happen if you skip this step.

Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...

Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment, plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 5) What does LB (-) plate contain? 6) What does LB/ amp (-) plate contain? 7) What does LB/amp (+) plate contain? 8) What does LB/amp/ara (+) plate contain?

Describe the selected environmental factor that affects children. Explain how the environmental factor you selected can...

Describe the selected environmental factor that affects children. Explain how the environmental factor you selected can potentially affect the health or safety of infants.

Please illustrate the steps to recombinant cloning of the vector to express c-Myc-GFP-His6 in mammalian cells...

Please illustrate the steps to recombinant cloning of the vector to express c-Myc-GFP-His6 in mammalian cells with detailed DNA sequences of primers. Please explain why the chosen particular vector was used and why the chosen version of the c-Myc gene was used.

Discuss the process of spore dispersal in Marchantia. What environmental factor stimulates the release of the...

Discuss the process of spore dispersal in Marchantia. What environmental factor stimulates the release of the spores? What are elaters? How do they function to release the spores?

Both F factor (a plasmid) and bacteriophages can be viewed as mobile genetic parasites. Describe some...

Both F factor (a plasmid) and bacteriophages can be viewed as mobile genetic parasites. Describe some similarities between these different genetic elements. What do these similarities suggest about the evolutionary history of plasmids and bacteriophages?

What are the common and useful features of plasmid

what is the purpose of the plasmid control pGAP? What is the of the genomic positive...

what is the purpose of the plasmid control pGAP? What is the of the genomic positive control gDNA? Why is exonuclease I added to the PCR products from the first round of amplification?

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