1.You are studying gene X, a methylation sensitive gene. You observe that when X is expressed,...

1.You are studying gene X, a methylation sensitive gene. You observe that when X is expressed, there is also a non-coding transcript, Y, that is expressed on the same chromosome.

–Outline experimental methods/techniques to investigate how Y can regulate the expression of gene X through methylation. Explain what each method would produce.

2.Explain what is meant by chromatin remodeling AND give an example of the molecular machinery involved.

Expert Solution

Q2) As far as the chromatin remodeling is concerned it the the highly energitic modification chromatin to allow way or acess of condensed genomic DNA to the regulatory transcription machinery proteins. such chromatin remodeling is principally carried out covalent histone modifications by specific enzymes, (e.g., histone acetyltransferases, methyltransferases, and kinases,) and ATP-dependent chromatin remodeling complexes which either move or restructure nucleosomes. Dynamic chromatin remodeling shares a epigenetic regulatory role in some key biological processes. Dynamic chromatin remodeling plays a role in cell death i.e, apoptosis, plays role in DNA replication and repair of DNA. Targeting chromatin remodeling plays a major role in treatment of several cancers. When chromatin is tightly packed, and not actively being transcribed it is called heterochromatin. When chromatin is loosely packed, and therefore accessible for transcription it is called euchromatin. ACF ATP-dependent chromatin assembly and remodeling factor, CHRAC chromatin accessibility complex glucocorticoid receptor are some examples of the molecular machinery involved.

gladiator answered 1 year ago

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