Q1: hydrolysis of an ester using a
Lipase CALB enzyme.
1. Use an organic chemistry textbook to draw the mechanism of
hydrolysis of the corresponding non enzymatic reaction-
ester hydrolysis using acid/base
catalysis. Enzymatic reactions often involve
general acid or general base
catalysis rather than such specific acid or
specific base catalysis. explain what this means.
3. With as much detail as you can identify the catalytic
residues involved in each step, and discuss their involvement in
the catalytic mechanism...