Question

Please use this paper to answer both the sub section question in detail please. this paper is avialbale online so thier is no plagirism

http://genesdev.cshlp.org/content/9/7/783.full.pdf

Q. What is cactSu? How was it discovered and what is the molecular change (in general terms) in CactSu comparing to the normal version of maternal Cactus?

Answer 1

Answer:

Q. What is Cact^Su? How was it discovered and what is the molecular change (in general terms) in Cact^Su comparing to the normal version of maternal Cactus?

• Cact^Su is a gain of function mutant allele of cactus
• Cactus is involved in the inhibition of the nuclear translocation of Dorsal protein by binding Dorsal and retaining in the the cytoplasm.
• Cact^Su allele is a strong dominant suppressor of ventrilization but it doesnot have any phenotype of its own and flies are fully viable with females producing wild type embryo. Cact^Su encodes a slightly truncated protein as compared to wild type Cactus protein and also appears more stable.
• Cact^Su/Cact^Su embryos 20% of the nuclei in the embryo circumferance had high levels of Dorsal. This percentage is reduced as compared to 27% in th wild-type embryos.
• It was discovered as a gain of function mutation (that were no longer able to suppress ea 831 and mapped to the same site as Su) during a genetic screen to revert the suppressing effect of the mutation.