Question

What is the General Secretory Pathway? (in detail). Also describe the secretion systems that depend on the GSP in order to deliver substrates to the periplasmic space and other areas.

Answer 1

The presence of the signal sequence i.e. a long stretch of primarily hydrophobic amino acids is the combining characteristics of all proteins, which are carried out of the gram-negative bacteria's cytoplasm by through general secretory pathway (GSP). The proteins having signal sequence and the cytoplasmic membrane interaction may be a natural event operated by the electrochemical energy potential over the cytoplasmic membrane, resulting in membrane integration. The large translocation of segment of hydrophilic polypeptide to the membranes periplasmic side mostly needs minimum six various proteins encoded by the sec genes always and is dependent on hydrolysis of ATP and the electrochemical energy potentia bothl. Precursors with a single, amino-terminal signal sequence processes by signal peptidase, permit them to be liberated into the periplasm, where they may persist or whence they may be placed into the outer membrane. For placed into the extracellular medium, selected proteins may also be carried across this membrane. Variety of different structural proteins secreated by vaious bacteria by a common pathway, mentioned here as the main GSP terminal branch.