Mycoplasmas are classified as Low G + C Gram-Positive Bacteria in the textbook. However their staining...

Mycoplasmas are classified as Low G + C Gram-Positive Bacteria in the textbook. However their staining phenotype indicates they are gram negative. What is the basis for classification as gram positive bacteria?

Expert Solution

Mycoplasma and its relatives have been classified in the phylum Firmicutes, consisting of low G+C Gram-positive bacteria such as Clostridium, Lactobacillus, and Streptococcus based on 16S rRNA gene analysis. Gram-positive organisms stain purple with a differential staining procedure developed in 1884 by Christian Gram. This procedure identifies cells that have a thick cell wall of peptidoglycan. Some firmicutes such as Mycoplasma have no cell wall. These are referred to as "low G+C" because their DNA typically has fewer G and C DNA bases than A and T bases as compared to other bacteria.

Therefore, inspite of not having a cell wall, mycoplasma are still classified as Gram-positive bacteria in the textbook because of their evolution from Gram-positive group of bacteria which have lost their cell wall, hence, based on 16S rRNA analysis, they are classified as gram positives along with other relatives of the phylum Firmicutes.

gladiator answered 1 year ago

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