In: Biology
The relationship between CO2 gas produced to sugar consumed is shown below.
m = n / 2s
where m is the number of moles of sugar consumed, n is the number of moles of CO2 produced, and s is the number of simple sugars in that sugar.
This means that for:
a monosaccharide, 2 CO2 molecules are produced per molecule of sugar
a disaccharide, 4 CO2 molecules are produced per molecule of sugar
a trisaccharide, 6 CO2 molecules are produced per molecule of sugar
Calculating the Rate of Respiration
First, use the Ideal Gas Law to convert the volume of gas to molecules. This is measured in moles, not the number of individual molecules. The Ideal Gas Law relates the moles of CO2 gas molecules to its volume as shown in the equation below.
PV = nRT
where P is the atmospheric pressure in the lab, V is the volume in liters, n is the number of moles of CO2, R is the gas constant 0.082 L-atm/mole-Kelvin, and T is the temperature in Kelvin.
Next, convert the moles of CO2 molecules produced to the moles of sugar consumed using the equation shown below.
m = n / 2s
Finally, combine several calculations to convert the results to milligrams of sugar fermented per minute:
convert from moles to grams
convert from grams to milligrams
divide by the length of respiration
The formula is below.
f = 1000mw / t
where f is the mg of sugar fermented per minute, m is the number of moles of sugar consumed, w is the molecular weight of the sugar in g/mole, and t is the respiration time in minutes.
For example, if 1 L of CO2 is collected when yeast is incubated with maltose for 5 minutes and the final temperature of the flask is 294.5 K, the milligrams of sugar fermented per minute are calculated as follows:
moles of CO2 = (1 atm × 1 L CO2) ÷ (0.082
L-atm/mole-Kelvin × 294.5 K)
moles of CO2 = 0.041 moles
moles of maltose consumed = 0.04141 moles of
CO2produced ÷ (2 × 2 simple sugars in maltose)
moles of maltose consumed = 0.01035 moles
mg of maltose per minute = (0.01035 moles maltose) × (MW of
maltose) × (1000 mg/g) ÷ (5 minutes)
mg of maltose per minute = 708.6 mg/min
Sugar Information Table | ||
---|---|---|
Sugar | Sugar Type | Molecular weight |
glucose | mono | 180.2 g/mole |
fructose | mono | 180.2 g/mole |
maltose | di | 342.3 g/mole |
maltotriose | tri | 504.4 g/mole |
For each of the sugars fermented by yeast, fill in the chart below to determine the volume of CO2 production.
Results Table(my answers)
Sugar |
Initial Gas |
Final Gas |
Volume of Co2 Produced |
glucose |
0.0 ml |
4.3 ml |
4.3 ml |
fructose |
0.0 ml |
1.3 ml |
1.3 ml |
maltose |
0.0 ml |
5.1 ml |
5.1 ml |
maltotriose |
0.0 ml |
1.0 ml |
1.0 ml |
For each of the sugars fermented by yeast, fill in the chart below to determine the mg of sugar consumed per minute during fermentation.
Calculations Table
Sugar |
MW (g/mole) |
Moles of CO2 produced |
Moles of Sugar consumed |
mg of sugar/min |
fructose |
||||
maltose |
||||
maltotriose |
My answers
Glucose temp 299.0k after 1 minute and 4.3ml in syringe
fructose temp 296.0k after 1 minute 1.3ml of gas in the syringe
maltose temp 300.0k after 1 minute 5.1 ml
maltotriose temp 295.6k after 1 minute 1.0 ml
Rate of respiration:
The measurement of respiration is very important because it provides a window through which we can determine the metabolic activity of plant tissues. During aerobic respiration, stored food (e.g., carbohydrates, fats, proteins) is combined with oxygen from the atmosphere to produce carbon dioxide, water and the energy needed to maintain the plant cell, tissue and quality of the commodity. The balanced equation for aerobic respiration is shown below.
C6H12O6 + 6O2 + 6H2O → 6CO2 + 12H2O + 673 kcal (38 ATP)
According to the question: the problem solved as follows:
The final result of the relation ship betweeen co2 and sugar consumption is as folows:
the result may be accurate or may it be surely related to question