Question

In: Biology

The relationship between CO2 gas produced to sugar consumed is shown below. m = n /...

The relationship between CO2 gas produced to sugar consumed is shown below.

m = n / 2s

where m is the number of moles of sugar consumed, n is the number of moles of CO2 produced, and s is the number of simple sugars in that sugar.

This means that for:

a monosaccharide, 2 CO2 molecules are produced per molecule of sugar

a disaccharide, 4 CO2 molecules are produced per molecule of sugar

a trisaccharide, 6 CO2 molecules are produced per molecule of sugar

Calculating the Rate of Respiration

First, use the Ideal Gas Law to convert the volume of gas to molecules. This is measured in moles, not the number of individual molecules. The Ideal Gas Law relates the moles of CO2 gas molecules to its volume as shown in the equation below.

PV = nRT

where P is the atmospheric pressure in the lab, V is the volume in liters, n is the number of moles of CO2, R is the gas constant 0.082 L-atm/mole-Kelvin, and T is the temperature in Kelvin.

Next, convert the moles of CO2 molecules produced to the moles of sugar consumed using the equation shown below.

m = n / 2s

Finally, combine several calculations to convert the results to milligrams of sugar fermented per minute:

convert from moles to grams

convert from grams to milligrams

divide by the length of respiration

The formula is below.

f = 1000mw / t

where f is the mg of sugar fermented per minute, m is the number of moles of sugar consumed, w is the molecular weight of the sugar in g/mole, and t is the respiration time in minutes.

For example, if 1 L of CO2 is collected when yeast is incubated with maltose for 5 minutes and the final temperature of the flask is 294.5 K, the milligrams of sugar fermented per minute are calculated as follows:

moles of CO2 = (1 atm × 1 L CO2) ÷ (0.082 L-atm/mole-Kelvin × 294.5 K)
moles of CO2 = 0.041 moles

moles of maltose consumed = 0.04141 moles of CO2produced ÷ (2 × 2 simple sugars in maltose)
moles of maltose consumed = 0.01035 moles

mg of maltose per minute = (0.01035 moles maltose) × (MW of maltose) × (1000 mg/g) ÷ (5 minutes)  
mg of maltose per minute = 708.6 mg/min

Sugar Information Table
Sugar Sugar Type Molecular weight
glucose mono 180.2 g/mole
fructose mono 180.2 g/mole
maltose di 342.3 g/mole
maltotriose tri 504.4 g/mole

For each of the sugars fermented by yeast, fill in the chart below to determine the volume of CO2 production.

Results Table(my answers)

Sugar

Initial Gas
Volume at t = 0 minutes (mL)

Final Gas
Volume at t = 1 minutes (mL)

Volume of Co2 Produced
Final - Initial (mL)

glucose

0.0 ml

4.3 ml

4.3 ml

fructose

0.0 ml

1.3 ml

1.3 ml

maltose

0.0 ml

5.1 ml

5.1 ml

maltotriose

0.0 ml

1.0 ml

1.0 ml

For each of the sugars fermented by yeast, fill in the chart below to determine the mg of sugar consumed per minute during fermentation.

Calculations Table

Sugar

MW (g/mole)

Moles of CO2 produced

Moles of Sugar consumed

mg of sugar/min

fructose

maltose

maltotriose

My answers

Glucose temp 299.0k after 1 minute and 4.3ml in syringe

fructose temp 296.0k after 1 minute 1.3ml of gas in the syringe

maltose temp 300.0k after 1 minute 5.1 ml

maltotriose temp 295.6k after 1 minute 1.0 ml

Solutions

Expert Solution

Rate of respiration:

The measurement of respiration is very important because it provides a window through which we can determine the metabolic activity of plant tissues. During aerobic respiration, stored food (e.g., carbohydrates, fats, proteins) is combined with oxygen from the atmosphere to produce carbon dioxide, water and the energy needed to maintain the plant cell, tissue and quality of the commodity. The balanced equation for aerobic respiration is shown below.

                   C6H12O6 + 6O2 + 6H2O → 6CO2 + 12H2O + 673 kcal (38 ATP)

According to the question: the problem solved as follows:

The final result of the relation ship betweeen co2 and sugar consumption is as folows:

the result may be accurate or may it be surely related to question


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