Chymotrypsin serves as a catalyst in the "Hydrolysis" of pepdite bonds - found in carboxylic groups...

Chymotrypsin serves as a catalyst in the "Hydrolysis" of pepdite bonds - found in carboxylic groups of amino acids, which consist of aromatic pedand groups and large hydrophobic groups. Chymotrypsin is also known to serve as a catalyst in the Hydrolysis of "ester - and amide bonds" , found in hydrophobic groups.

1. Would you expect the K_Mof the reactions of Chymotrypsin with the different substrates to have the same value ? explain your reasoning.

2. Would you expect the V_maxof the reactions of Chymotrypsin with the different substrates to have the same value ? explain your reasoning.

3. Would you expect the " pH-dependence " of the "hydrolysis - reactions" of the different substrates to be the same for all substrates ? explain your reasoning.

Expert Solution

1. michaelis menten equation for the chymotrypsin catalysed hydrolysis of peptide bond is

V = Vmax[S]/(Km+[S])

Where Km = K_-1+K₂/ K₁

From the equation of Km it is clear that the MM constant is independent of concentration of substrate, so the reaction of chymotrypsin with different substrate will have same value.

2. Vmax = K[Et]

Where [Et] = [E] + [ES]

Since Vmax depends upon concentration of ES complex formed. Different substrate will have different ES complex concentration. So Vmax will vary with change in substrate.

3. the " pH-dependence" of the "hydrolysis - reactions" of the different substrates will be the same for all substrates because the MM equation depends upon substrate concentration and not directly on species causing hydroslysis. The pH dependance will only vary the activity of chymotrypsin which will be same for all substrates.

queen_honey_blossom answered 2 years ago

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