Outline requirements for correct protein folding

Outline requirements for correct protein folding

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gladiator answered 1 year ago

Outline how protein folding occurs and how this process is reversed to facilitate digestion with proteases...

Outline how protein folding occurs and how this process is reversed to facilitate digestion with proteases such as trypsin prior to mass spectrometry analysis.

The hydrophobic effect is the primary driving force for protein folding because A) a folded protein...

The hydrophobic effect is the primary driving force for protein folding because A) a folded protein maximizes the entropy of water B) an unfolded protein maximizes the entropy of a biological system C) a folded protein is able to form the most hydrogen bonds D) hydrogen bonds within the protein replace hydrogen bonds between the protein and water

7. Discuss the primary issues associated with protein folding.

Describe in comeplete detail the anfinsen experiment in protein folding.

Describe the likely order of events for the folding of a globular protein and why is...

Describe the likely order of events for the folding of a globular protein and why is this sequence of events likely? What is the chemical/physical rationale for each step? Include a brief discussion of Levinthal’s paradox.

How do the 4 levels of protein folding relate to one another?

We discussed that the main driving force for protein folding (in an aqueous environment) is the...

We discussed that the main driving force for protein folding (in an aqueous environment) is the hydrophobic effect. Using a thermodynamic analysis explain why this is the case. You analysis should take in to account the peptide bonds, polar side chains, non-polar side chains, the influence of solvent water, and the enthaplic and entropic contributions of each of these.

23. What role does aberrant protein folding play in the development of a disease such as...

23. What role does aberrant protein folding play in the development of a disease such as Alzheimer’s disease?

In the Anfinsen protein folding experiment the sequence of removing urea or BME makes a difference...

In the Anfinsen protein folding experiment the sequence of removing urea or BME makes a difference for functional refolding of the protein. Explain why removing BME first leads to a nonfunctional enzyme, but removing urea first leads to a functional enzyme. Why does adding small amounts of BME alone to the solution with the nonfunctional enzyme "rescue" the function biochemistry

Briefly describe the process of protein folding. In your description comment on the various models that...

Briefly describe the process of protein folding. In your description comment on the various models that help describe protein folding, the value of using a “Folding Funnel” to describe the folding process, and the role of chaperones and chaperoning in in vivo folding.

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