Question

Application of CRISPR technology in dairy food production. In other words, how to solve phage problems using CRIPSR-based immunity?

Answer 1

Clustered regulatory Interspaced Short Palindromic Repeats (CRISPR) is segments of short prokaryotic base sequences. The CRISPR system is found in many bacteria and has been shown to be involved in bacterial acquired immunity to viruses (adaptive immunity).

In the field of biotechnology, CRISPR is used for genome editing of microorganisms and human embryos as well. Editing the plant genome with the preassembled CRISPER-Cs9 (does not include the introduction of foreign DNA) and directing the RNA into protoplasts of plant cells is helpful in inducing targeted mutagenesis. This is usually applied to achieve required phenotypic characters in plants.

Uses in dairy food production:-
-Cas enzymes are under study. First Cas9 and then cas 13.
- Cas enzymes in bacteria are being used to enhance the protein content, shelf life and flavors of cheese and creamy yogurts.
- act as the preservation of active bacterial cultures used in the manufacture of dairy products.
- CRISPER tools are faster and cheaper has got various food and dairy companies interested in implementing them.