Which of the following are true concerning the limit of quantification (LOQ) of a particular analysis...

Which of the following are true concerning the limit of quantification (LOQ) of a particular analysis method? Select all that are valid.

	LOQ is larger than the detection limit
	LOQ depends on detector sensitivity
	LOQ is equal to 10 times the calibration line slope
	LOQ = detection limit

Expert Solution

Correct option. a. and b.

LOD: The lowest concentration of a molecule that can be detected by the specified analytical method.

LOQ: The lowest concentration of a molecule that can be quantified by the specified analytical method.

Consider an example- A hormone assay by ELISA method. A positive color change indicates that the specified molecule (hormone) is present in the sample. However, the same color change can’t be quantified appropriately using spectrophotometer whereas ELISA reader is suited for it.

Thus-

1. LOD (seeing positive color change when a minimum [hormone] is present in sample) may NOT always be quantified (using spectrophotometer); Or LOD may be lower than LOQ.

Thus, option d. LOQ = detection limit may NOT always hold true.

2. LOQ depends on the defector’s sensitivity (ELISA reader is more sensitive than spectrophotometer).

Option c. Not always true. Depends on the type of method/ instrumentation.

queen_honey_blossom answered 1 year ago

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