Question

1. Select statements that are true regarding the function of the protein with the sequence provided below: MKSKILYGPSPVYPLESGTGGEHIHKYISKYAKIPGCVAMTNAHTKENLFYAELVELTCKLAHSLKRYGVTQESTIAVCSENSLQYFIPMISGLYIGAVTAAVSDKYDERELISCLNLSKPSLVFCSQQTLAKILQVKPKLDYIKKILVLDSVDNVDSMQSMHNLILKHCHPDFDVKHFKPDSFNDQKVAMILNSPGSTGLPKGVMLTHKNLLVRFCHCKDPIFGNQISPGTAILTAIPFPHGFGMSTVLGYLTCGFRIVLMHTFDSQLYLQSLQDYNVESTVMVPTLMTFFAKSPLVENYHLPNLKEIASGGAPLSKEIGEAVAKRFKLRTIRQGYGLTETTSTILITAAGEVVPGSTGRLVPFFAAKVVDCTSGEALGSNEVGELYFKGDMNMKGYCNNPEATAEMIDNDGWLHSGDLGYYNETGHFFIVDRLKSGIKYKGYQVAPAELEGILLTHPSIMDAGVTGIPDEYAGELPAACVVLKPGHNITEKSLVNYVSSRVSSVKKLRGGVRFVDEIPKGSTGKIDTRALKEILQKPKSHL *

• It is an enzyme.
• The sequence is an exact match to Aquatica lateralis.
• The substrate for the enzyme is luciferin.
• The substrate for the enzyme is luciferase.
• The protein is found in firefly species.
• The protein sequence is not an exact match to any known firefly species.
• The sequence is from the Kampung Kuantan fireflies.
• The protein is involved in bioluminescence.
• The protein is a fluorescent protein.

2. For the protein sequence provided below; identify the function of the protein. Select other statements that are TRUE regarding that protein sequence. //Protein sequence: MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT *

• The protein is to be able to interact with an enzyme called Angiotensin Converting Enzyme 2.
• The protein is a recognizable feature that gives the name to a specific group of related viruses when the virus is studied using electron microscopy.
• The protein is called a corona protein and is composed of lipid chains that is the reason why coronaviruses can be degraded by soap.
• There are no available structures of this protein.
• Homologs of this protein can also be found in bats.

Answer 1

1. From the BLAST search tool, It is found that the given sequence is of the enzyme luciferase that is found to be present in firefly aquatica lateralis. It utilizes luciferin as a substrate and generates bioluminescence in an ATP-dependent reaction. It matches with the sequence found in aquatica lateralis with the maximum identity (i.e. 74%).

So, the correct options are:

• It is an enzyme.
• The sequence is an exact match to Aquatica lateralis.
• The substrate for the enzyme is luciferin.
• The protein is found in firefly species.
• The protein is involved in bioluminescence.

2. The given peptide sequence is of surface glycoprotein present in the SARS-COV 2. It is also known as corona spike protein as it is found to be present in the spikes of the virus, responsible for interacting with the Angiotensin Converting Enzyme 2 (ACE-2) receptors on the cell for entry.  Also, It has structural similarity to that of the spike protein present in bats.

Hence, the correct options are:

• The protein is to be able to interact with an enzyme called Angiotensin Converting Enzyme 2.
• The protein is a recognizable feature that gives the name to a specific group of related viruses when the virus is studied using electron microscopy.
• Homologs of this protein can also be found in bats.