An enzyme normally functions at pH 4. At pH 8, it is denatured. How can a...

An enzyme normally functions at pH 4. At pH 8, it is denatured. How can a change in pH cause denaturation in a protein composed of more than one polypeptide? The change in hydrogen ion concentration can affect enzyme structure and function by __________.

	A.	breaking peptide bonds between amino acids in the polypeptide
	B.	disrupting hydrogen bonds between amino anc carboxyl residues in the backbone
	C.	disrupting hydrogen bonds and ionic bonds between R-groups in the polypeptide
	D.	disrupting hydrogen bonding and ionic bonding between polypeptides
	E.	doing all of the above
	F.	doing B, C, and D only

Solutions

Expert Solution

A peptide bond is a covalent bond. Covalent bonds are not broken if there is a change in pH. So, option A is wrong.

Change in pH can break the hydrogen bond between the amino and carboxyl residues in the backbone. So, option B is correct

Change in pH can break the hydrogen bond between disrupting hydrogen bonds and ionic bonds between R-groups in the polypeptide. Due to change in pH, the COOH and NH2 group present in the side chain changes their ionic form that results in the breakage of hydrogen bond

Change in pH can break the hydrogen bond. Change in pH also breaks the ionic bond. As the pH changes, the charge on the COOH and NH2 group present in the side chain also changes. Since ionic bond required a positive and a negatively charged group, so the ionic bond is broken due to change in pH.

So correct answer is option F. doing B, C, and D only

gladiator answered 1 year ago

Next > < Previous

Related Solutions

An enzyme has a broad pH optimum having high rates of activity between pH 4 and...

An enzyme has a broad pH optimum having high rates of activity between pH 4 and 12. X-ray diffraction studies show that the enzyme has an active site with glutamate and arginine residues in close proximity to the substrate. Explain how these two residues might be able to promote concerted acid-base catalysis (which residue is acting as the acid, which is acting as the base). Finally, explain why enzyme activity drops off sharply below pH 4 and above pH 12.

How do changes in pH affect the activity of an enzyme?

How do changes in pH affect the activity of an enzyme? disrupt hydrogen and ionic bonds disrupt peptide bonds disrupt ALL bonds enzymes are NOT sensitive to pH changes When DNS is added to your tubes, in the amylase activity experiment, what becomes reduced and what oxidized? maltose is reduced and DNS is oxidized maltose is oxidized and DNS is reduced starch is reduced and...

4. Papain is a protease with a pH optimum of approximately 6.5. The enzyme utilizes a...

4. Papain is a protease with a pH optimum of approximately 6.5. The enzyme utilizes a catalytic triad to break peptide bonds. The nucleophile within this triad is a cysteine (Cys25) having a perturbed pKa value of 3.3. Write the Henderson-Hasselbalch equation. Using this equation, calculate the percent deprotonated of papain’s Cys25 side chain at pH 3.7. Why will the enzyme not function maximally at this pH? There will be no partial credit for the calculation, but your work must...

Enzyme A has an optimal pH of 4.5, while enzyme B has an optimal pH of...

Enzyme A has an optimal pH of 4.5, while enzyme B has an optimal pH of 7.2. If you think about the tertiary structure of these two enzymes in their optimal pH environments, which would you say has the fewest positively charged R groups in the primary amino acid sequence? Group of answer choices: enzyme A ,both enzymes could have equal numbers of positively charged R groups, enzyme B

4. The rate with which enzymes can transform a substrate varies from enzyme to enzyme, and...

4. The rate with which enzymes can transform a substrate varies from enzyme to enzyme, and sometimes substrate to substrate. a) How are competitive and uncompetitive reversible inhibition similar? How is their effect on rate different? b) Enzyme reaction rates drop off dramatically as temperatures pass 50 °C or so, and do not return to their previous rate when cooled. Explain the drop off in activity and the lack of return to the previously higher reaction rates after heating. c)...

If changing the pH of an enzyme solution greatly reduces the activity of that enzyme without...

If changing the pH of an enzyme solution greatly reduces the activity of that enzyme without precipitation, how could you tell in the lab if the result of denaturation or just going to a pH that is just not a good one for the enzyme? And how would a change in pH impact or even stop enzyme function without denaturation? (consider the enzymes that are active in your stomach, such as pepsin)

1) a)draw the structure of histidine at pH 4 and at pH 8. Make sure you...

1) a)draw the structure of histidine at pH 4 and at pH 8. Make sure you clearly indicate all charges. b)Briefly describe the nature of the H-bonds that stabilize parallel and antiparallel -sheets. How do the H-bonds in these two types of secondary structure differ? You can draw simple diagrams if you wish, but there is no need for detailed structures.

How does the Near Attack Conformation (NAC) contribute to enzyme function? How can an enzyme put...

How does the Near Attack Conformation (NAC) contribute to enzyme function? How can an enzyme put a substrate into an energetically unfavorable conformation? How does modern drug design utilize NAC? Please go into detail, provide examples, and use diagrams to help illustrate your explanation. Especially use diagrams please. NAC means "Near Attack Conformation". I am struggling to understand how all of these relate. Thank you so much for the help!

How does temperature and pH affect the ability of an enzyme to bind substrate? and what...

How does temperature and pH affect the ability of an enzyme to bind substrate? and what specific changes occur to the structure of an enzyme when it is boiled?

4. How many different ways can you put 8 balls in 8 boxes A1, . ....

4. How many different ways can you put 8 balls in 8 boxes A1, . . . , A8 if (a) the balls are all different and no box is empty 8! (b) the balls are all different and only three boxes A1, A2 and A3 are empty (c) the balls are all different and exactly four boxes are empty (d) the balls are all different and each box is either empty or contains exactly two balls (e) the balls...

Subjects