An enzyme normally functions at pH 4. At pH 8, it is denatured. How can a...

An enzyme normally functions at pH 4. At pH 8, it is denatured. How can a change in pH cause denaturation in a protein composed of more than one polypeptide? The change in hydrogen ion concentration can affect enzyme structure and function by __________.

	A.	breaking peptide bonds between amino acids in the polypeptide
	B.	disrupting hydrogen bonds between amino anc carboxyl residues in the backbone
	C.	disrupting hydrogen bonds and ionic bonds between R-groups in the polypeptide
	D.	disrupting hydrogen bonding and ionic bonding between polypeptides
	E.	doing all of the above
	F.	doing B, C, and D only

Expert Solution

A peptide bond is a covalent bond. Covalent bonds are not broken if there is a change in pH. So, option A is wrong.

Change in pH can break the hydrogen bond between the amino and carboxyl residues in the backbone. So, option B is correct

Change in pH can break the hydrogen bond between disrupting hydrogen bonds and ionic bonds between R-groups in the polypeptide. Due to change in pH, the COOH and NH2 group present in the side chain changes their ionic form that results in the breakage of hydrogen bond

Change in pH can break the hydrogen bond. Change in pH also breaks the ionic bond. As the pH changes, the charge on the COOH and NH2 group present in the side chain also changes. Since ionic bond required a positive and a negatively charged group, so the ionic bond is broken due to change in pH.

So correct answer is option F. doing B, C, and D only

gladiator answered 2 years ago

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