In: Biology
Temperature is an important factor which impacts reaction rate.when temperature increases the rate of reaction increases this is because of the disproportionately large increase in the number of high energy collisions .For many reactions happening at around room temperature ,the rate of reaction doubles for every 10 degree rise in temperature.In an enzymatic reaction raising temperature speeds up reaction.extreme high temperature causes enzyme to lose its shape.
Experimental explanation
Label test tubes -8 for catalase and with each temperature and the other 8 for hydrogen peroxide.place the boiling tubes in the test tube tracks.add 1 drop of catalase enzyme intoa catalase tube labelled Ooc.add 1 ml of hrogen peroxide to the hydrogen peroxide tube labelled Ooc.place two boiling tubes -one catalase and one hydrogen peroxide into the ooc water bath for 10 minutes to allow for the equillibrium of temperature.add the hydrogen peroxide catalase solution and start stop clock.measure the bubble after 30 seconds and record in table.repeat above steps for different values of increase in temperature.plot graph with height of bubble column in 30 seconds against the temperature of each temperature.determine optimum temperature.We can see an increase in slope or rate of reaction before optimal temperature is obtained. this is because when temperature increases the kinetic energy and number of random collisions of enzyme with substrate increases per unit time.With the increase in frequency in the number of collisions,the probability of substrate colliding the correct orientation also increases .Hence rate of reaction with temperature occurs,up until optimum temperature is attained.